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Literature summary for 1.14.13.154 extracted from

  • Lambalot, R.H.; Cane, D.E., Aparicio, J.J.; Katz, L.
    Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK (1995), Biochemistry, 34, 1858-1866.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overproduction of EryK in Escherichia coli as insoluble inclusion bodies Saccharopolyspora erythraea

Inhibitors

Inhibitors Comment Organism Structure
erythromycin D substrate inhibition Saccharopolyspora erythraea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
erythromycin D pH 7.5, 30°C Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
erythromycin D + NADPH + H+ + O2 Saccharopolyspora erythraea
-
erythromycin C + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
erythromycin B + NADPH + H+ + O2 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B Saccharopolyspora erythraea erythromycin A + NADP+ + H2O
-
?
erythromycin D + NADPH + H+ + O2
-
Saccharopolyspora erythraea erythromycin C + NADP+ + H2O
-
?
erythromycin D + NADPH + H+ + O2 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B Saccharopolyspora erythraea erythromycin C + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
EryK
-
Saccharopolyspora erythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharopolyspora erythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
erythromycin D pH 7.5, 30°C Saccharopolyspora erythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharopolyspora erythraea

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 a heme-thiolate protein (P-450) Saccharopolyspora erythraea
NADPH
-
Saccharopolyspora erythraea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0166
-
erythromycin D pH 7.5, 30°C Saccharopolyspora erythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
225
-
erythromycin D pH 7.5, 30°C Saccharopolyspora erythraea