Cloned (Comment) | Organism |
---|---|
overproduction of EryK in Escherichia coli as insoluble inclusion bodies | Saccharopolyspora erythraea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythromycin D | substrate inhibition | Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
erythromycin D + NADPH + H+ + O2 | Saccharopolyspora erythraea | - |
erythromycin C + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
erythromycin B + NADPH + H+ + O2 | 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B | Saccharopolyspora erythraea | erythromycin A + NADP+ + H2O | - |
? | |
erythromycin D + NADPH + H+ + O2 | - |
Saccharopolyspora erythraea | erythromycin C + NADP+ + H2O | - |
? | |
erythromycin D + NADPH + H+ + O2 | 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B | Saccharopolyspora erythraea | erythromycin C + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EryK | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a heme-thiolate protein (P-450) | Saccharopolyspora erythraea | |
NADPH | - |
Saccharopolyspora erythraea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0166 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
225 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea |