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Literature summary for 1.14.13.14 extracted from
- The 2-hydroxylation of trans-cinnamic acid by chloroplasts from Melilotus alba Desr (1974), Arch. Biochem. Biophys., 163, 617-624.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose 6-phosphate | 400% activation at 4 mM | Melilotus albus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | lamellar, 50% of the hydroxylase activity | Melilotus albus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-cinnamate + NADPH + O2 | Melilotus albus | - |
2-hydroxycinnamate + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Melilotus albus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Melilotus albus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0004 | - |
supernatant activity | Melilotus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-cinnamate + NADPH + O2 | - |
Melilotus albus | 2-hydroxycinnamate + NADP+ + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Melilotus albus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | less effective than NADPH | Melilotus albus | |
| NADPH | - |
Melilotus albus | |
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