Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.128 extracted from

  • Summers, R.M.; Louie, T.M.; Yu, C.L.; Gakhar, L.; Louie, K.C.; Subramanian, M.
    Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids (2012), J. Bacteriol., 194, 2041-2049.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron a Rieske nonheme iron monooxygenase. The enzyme contains 2 mol of iron per mol of monomer Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-methylxanthine + O2 + NADH + H+ Pseudomonas putida specific substrate xanthine + NAD+ + H2O + formaldehyde
-
?
7-methylxanthine + O2 + NADH + H+ Pseudomonas putida CBB5 specific substrate xanthine + NAD+ + H2O + formaldehyde
-
?
additional information Pseudomonas putida caffeine, paraxanthine, and theobromine are not demethylated by the enzyme ?
-
?
additional information Pseudomonas putida CBB5 caffeine, paraxanthine, and theobromine are not demethylated by the enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida M1EY73
-
-
Pseudomonas putida CBB5 M1EY73
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylxanthine + O2 + NADH + H+ specific substrate Pseudomonas putida xanthine + NAD+ + H2O + formaldehyde
-
?
7-methylxanthine + O2 + NADH + H+ specific substrate Pseudomonas putida CBB5 xanthine + NAD+ + H2O + formaldehyde
-
?
additional information caffeine, paraxanthine, and theobromine are not demethylated by the enzyme Pseudomonas putida ?
-
?
additional information caffeine, paraxanthine, and theobromine are not demethylated by the enzyme Pseudomonas putida CBB5 ?
-
?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
7-methylxanthine-specific N-demethylase
-
Pseudomonas putida
methylxanthine N-demethylase
-
Pseudomonas putida
NdmC
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida