Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | a Rieske nonheme iron monooxygenase. The enzyme contains 2 mol of iron per mol of monomer | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylxanthine + O2 + NADH + H+ | Pseudomonas putida | specific substrate | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
7-methylxanthine + O2 + NADH + H+ | Pseudomonas putida CBB5 | specific substrate | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
additional information | Pseudomonas putida | caffeine, paraxanthine, and theobromine are not demethylated by the enzyme | ? | - |
? | |
additional information | Pseudomonas putida CBB5 | caffeine, paraxanthine, and theobromine are not demethylated by the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | M1EY73 | - |
- |
Pseudomonas putida CBB5 | M1EY73 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylxanthine + O2 + NADH + H+ | specific substrate | Pseudomonas putida | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
7-methylxanthine + O2 + NADH + H+ | specific substrate | Pseudomonas putida CBB5 | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
additional information | caffeine, paraxanthine, and theobromine are not demethylated by the enzyme | Pseudomonas putida | ? | - |
? | |
additional information | caffeine, paraxanthine, and theobromine are not demethylated by the enzyme | Pseudomonas putida CBB5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
7-methylxanthine-specific N-demethylase | - |
Pseudomonas putida |
methylxanthine N-demethylase | - |
Pseudomonas putida |
NdmC | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida |