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Literature summary for 1.14.13.122 extracted from

  • Reinbothe, S.; Bartsch, S.; Rossig, C.; Davis, M.Y.; Yuan, S.; Reinbothe, C.; Gray, J.
    A protochlorophyllide (Pchlide) a oxygenase for plant viability (2019), Front. Plant Sci., 10, 593 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.122

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A247D 25.0% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
A247D 31.2% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
C127A 22% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
C147A 36.4% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
F286A 0.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
F337A 20% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
F417A 0% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H129A 25.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H150A 24.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H248A 29.5%drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H248A/H253A 88% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H253A 41.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
H386A 0% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
L440A 0% drop in protochlorophyllide a-oxygenase activityy in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
N399A 0% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
P354A 94.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
P441A 19% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
R390A 0.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
V249A 7.0% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
W351A 15.5% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana
W400A 0.75% drop in protochlorophyllide a-oxygenase activity in the generated mutant proteins relative to the wild-type activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
inner plastid envelope
-
 Arabidopsis thaliana
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61300
-
 calculated from sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8W496
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+
-
 Arabidopsis thaliana chlorophyllide b + 3 H2O + 2 NADP+
-
 ?

Synonyms

Synonyms Comment Organism
AtPTC52
-
 Arabidopsis thaliana
CAO
-
 Arabidopsis thaliana
Pchlide a oxygenase
-
 Arabidopsis thaliana
protochlorophyllide a oxygenase
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana
-
-
 8.9