Cloned (Comment) | Organism |
---|---|
gene salA, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Pseudomonas reinekei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0024 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0028 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0038 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0096 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0127 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0158 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0158 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
50712 | - |
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylsalicylate + NADH + 2 H+ + O2 | Pseudomonas reinekei | - |
4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
5-methylsalicylate + NADH + 2 H+ + O2 | Pseudomonas reinekei | - |
5-methylcatechol + NAD+ + H2O + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas reinekei | Q0VH44 | gene salA | - |
Purification (Comment) | Organism |
---|---|
native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration | Pseudomonas reinekei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-methylsalicylate-grown cell | - |
Pseudomonas reinekei | - |
culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
culture condition:5-methylsalicylate-grown cell | - |
Pseudomonas reinekei | - |
culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
purified enzyme from 4-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
90 | - |
purified enzyme from 5-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
120 | - |
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 3-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
3-methylsalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 3-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
4-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 4-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
4-methylsalicylate + NADH + 2 H+ + O2 | - |
Pseudomonas reinekei | 4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
5-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 5-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
5-methylsalicylate + NADH + 2 H+ + O2 | - |
Pseudomonas reinekei | 5-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
salicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | catechol + NAD+ + H2O + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
Synonyms | Comment | Organism |
---|---|---|
salicylate 1-hydroxylase | - |
Pseudomonas reinekei |
salicylate hydroxylase | - |
Pseudomonas reinekei |
SalOH | - |
Pseudomonas reinekei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas reinekei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.6 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
2.2 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
2.9 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
4.6 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
5.4 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
6.4 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
7.8 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas reinekei |
Organism | Comment | Expression |
---|---|---|
Pseudomonas reinekei | 5-chlorosalicylate induces the enzyme | up |
General Information | Comment | Organism |
---|---|---|
metabolism | salicylate 1-hydroxylase is not clustered with the meta cleavage pathway | Pseudomonas reinekei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
340 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
340 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
410 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
480 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1000 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
2700 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
3300 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei |