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Literature summary for 1.14.13.1 extracted from

  • Camara, B.; Bielecki, P.; Kaminski, F.; dos Santos, V.M.; Plumeier, I.; Nikodem, P.; Pieper, D.H.
    A gene cluster involved in degradation of substituted salicylates via ortho cleavage in Pseudomonas sp. strain MT1 encodes enzymes specifically adapted for transformation of 4-methylcatechol and 3-methylmuconate (2007), J. Bacteriol., 189, 1664-1674.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene salA, DNA and amino acid sequence determination and analysis, phylogenetic analysis Pseudomonas reinekei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0024
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0028
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0038
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0096
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0127
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
0.0158
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
0.0158
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei
50712
-
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylsalicylate + NADH + 2 H+ + O2 Pseudomonas reinekei
-
4-methylcatechol + NAD+ + H2O + CO2
-
?
5-methylsalicylate + NADH + 2 H+ + O2 Pseudomonas reinekei
-
5-methylcatechol + NAD+ + H2O + CO2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas reinekei Q0VH44 gene salA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration Pseudomonas reinekei

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-methylsalicylate-grown cell
-
Pseudomonas reinekei
-
culture condition:5-chlorosalicylate-grown cell
-
Pseudomonas reinekei
-
culture condition:5-methylsalicylate-grown cell
-
Pseudomonas reinekei
-
culture condition:salicylate-grown cell
-
Pseudomonas reinekei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
purified enzyme from 4-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
90
-
purified enzyme from 5-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
120
-
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 3-chlorocatechol + NAD+ + H2O + CO2
-
?
3-methylsalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 3-methylcatechol + NAD+ + H2O + CO2
-
?
4-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 4-chlorocatechol + NAD+ + H2O + CO2
-
?
4-methylsalicylate + NADH + 2 H+ + O2
-
Pseudomonas reinekei 4-methylcatechol + NAD+ + H2O + CO2
-
?
5-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 5-chlorocatechol + NAD+ + H2O + CO2
-
?
5-methylsalicylate + NADH + 2 H+ + O2
-
Pseudomonas reinekei 5-methylcatechol + NAD+ + H2O + CO2
-
?
salicylate + NADH + H+ + O2
-
Pseudomonas reinekei catechol + NAD+ + H2O + CO2
-
?

Subunits

Subunits Comment Organism
? x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei

Synonyms

Synonyms Comment Organism
salicylate 1-hydroxylase
-
Pseudomonas reinekei
salicylate hydroxylase
-
Pseudomonas reinekei
SalOH
-
Pseudomonas reinekei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas reinekei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.6
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
2.2
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
2.9
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
4.6
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
5.4
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
6.4
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
7.8
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas reinekei

Expression

Organism Comment Expression
Pseudomonas reinekei 5-chlorosalicylate induces the enzyme up

General Information

General Information Comment Organism
metabolism salicylate 1-hydroxylase is not clustered with the meta cleavage pathway Pseudomonas reinekei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
340
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
340
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
410
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
480
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1000
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
2700
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
3300
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei