General Stability | Organism |
---|---|
dithiothreitol, 0.5 mM, stabilizes | Cutaneotrichosporon cutaneum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | - |
Cutaneotrichosporon cutaneum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cutaneotrichosporon cutaneum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Cutaneotrichosporon cutaneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43400 | 45300 | gel filtration, SDS-PAGE | Cutaneotrichosporon cutaneum |
45300 | - |
1 * 45300 SDS-PAGE | Cutaneotrichosporon cutaneum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cutaneotrichosporon cutaneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cutaneotrichosporon cutaneum |
Storage Stability | Organism |
---|---|
4°C, 0.5 mM dithiothreitol, 10% loss of activity after 1 week | Cutaneotrichosporon cutaneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | pyrogallol + CO2 + H2O + NAD+ | - |
? | |
2,4-dihydroxybenzoate + NAD(P)H + O2 | - |
Cutaneotrichosporon cutaneum | benzene-1,2,4-triol + CO2 + H2O + NAD(P)+ | - |
? | |
2,5-dihydroxybenzoate + NADH + O2 | or NADPH | Cutaneotrichosporon cutaneum | benzene-1,2,5-triol + CO2 + H2O + NAD+ | - |
? | |
2,6-dihydroxybenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | pyrogallol + CO2 + H2O + NAD+ | - |
? | |
4-aminosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 4-aminocatechol + CO2 + NAD+ + H2O | - |
? | |
4-chlorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-4-chlorobenzene + CO2 + NAD+ + H2O | - |
? | |
5-aminosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 5-aminocatechol + CO2 + NAD+ + H2O | - |
? | |
5-chlorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-chlorobenzene + CO2 + NAD+ + H2O | - |
? | |
5-fluorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-fluorobenzene + CO2 + NAD+ + H2O | - |
? | |
5-methoxysalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-methoxybenzene + CO2 + NAD+ + H2O | - |
? | |
5-methylsalicylate + NADH + H+ + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O | - |
? | |
salicylate + NADH + H+ + O2 | - |
Cutaneotrichosporon cutaneum | catechol + NAD+ + H2O + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45300 SDS-PAGE | Cutaneotrichosporon cutaneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Cutaneotrichosporon cutaneum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | about 75% of activity maximum at pH 6.5 and 8.5 | Cutaneotrichosporon cutaneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
NADH | - |
Cutaneotrichosporon cutaneum | |
NADPH | 60% of the activity with NADH | Cutaneotrichosporon cutaneum |