Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
apoenzyme is crystallized by dialysis method, using ammonium sulfate as the precipitant | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
K163E | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
K163G | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
K163R | site directed mutagenesis, Lys163 is involved in the NADH-binding site | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | FAD, salicylate and NADH, comparison of wild-type, recombinant and mutant enzyme | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
recombinant native and mutant protein, expressed in E. coli, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
salicylate + NADH + H+ + O2 | - |
Pseudomonas putida | catechol + NAD+ + H2O + CO2 | - |
? |