General Stability | Organism |
---|---|
75% loss of activity after 16 h dialysis | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 34% inhibition | Pseudomonas sp. | |
EDTA | 1 mM, 32% inhibition | Pseudomonas sp. | |
iodoacetate | 0.01 mM, 77% inhibition | Pseudomonas sp. | |
KCN | 1 mM, 52% inhibition | Pseudomonas sp. | |
NEM | 1 mM, 38% inhibition | Pseudomonas sp. | |
NH4+ | inhibition of activity of reductase component | Pseudomonas sp. | |
PCMB | 0.005 mM, complete inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
ferrocytochrome c | - |
Pseudomonas sp. | |
0.0063 | - |
reduced 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | |
0.032 | - |
NADH | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | reductase component contains an iron-sulfur cluster of the [2Fe-2S]-type, contains 2.1 mol of iron and 1.7 mol of acid-labile sulfide per mol of reductase | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. CBS 3 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
reductase component loses activity in presence of molecular oxygen | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
reductase component | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorophenylacetate + ferrocytochrome c + H+ + O2 | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c | - |
r | |
4-chlorophenylacetate + ferrocytochrome c + H+ + O2 | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c | - |
r | |
4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2 | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol | - |
r | |
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2 | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol | - |
r | |
additional information | the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium | Pseudomonas sp. | ? | - |
? | |
additional information | the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium | Pseudomonas sp. CBS 3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE | Pseudomonas sp. |
More | the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
3 min, 50% loss of activity | Pseudomonas sp. |
55 | - |
90-100% loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 4°C, 10 days, 50% loss of activity | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | 30% as effective as NADH | Pseudomonas sp. |