KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
O2 | - |
Burkholderia cepacia | |
0.3 | - |
Phthalate | 25°C | Burkholderia cepacia | |
0.5 | - |
Phthalate | 4°C | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | one Fe2+ is bound at a mononuclear site | Burkholderia cepacia | |
Iron | two iron atoms are bound in a Rieske-type [2Fe-2S] center | Burkholderia cepacia | |
additional information | optimal activity at ionic strength of 80 mM | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
4 * 48000, SDS-PAGE | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
DB01 | - |
Burkholderia cepacia | - |
two separate proteins are required for phthalate dioxygenase activity: phthalate dioxygenase and phthalate dioxygenase reductase | - |
Burkholderia cepacia DB01 | - |
DB01 | - |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phthalate + NADH + H+ + O2 = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ | reduced phthalate dioxygenase reductase first interacts with phthylate dioxygenase and reduces the Rieske [2Fe-2S]center. Phthalate and O2 then bind reversibly and reduced phthalate dioxygenase transfers one more electron to the enzyme | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.31 | - |
- |
Burkholderia cepacia |
Storage Stability | Organism |
---|---|
4°C, pH 8.0, 100 mM HEPES buffer, 20% v/v glycerol, stable for 1 week | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phthalate + NADH + O2 | - |
Burkholderia cepacia | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? | |
phthalate + NADH + O2 | - |
Burkholderia cepacia DB01 | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 48000, SDS-PAGE | Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
pH 6.8 or pH 8.0, 12 h stable | Burkholderia cepacia |
42 | - |
1 h, stable | Burkholderia cepacia |
60 | - |
5 min, stable | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.2 | - |
Burkholderia cepacia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
room temperature, 12 h, stable | Burkholderia cepacia |
8 | - |
room temperature, 12 h, stable | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | specific for NADH | Burkholderia cepacia |