Literature summary for 1.14.12.23 extracted from

Singh, D.; Kumari, A.; Ramaswamy, S.; Ramanathan, G.
Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2 (2014), Biochem. Biophys. Res. Commun., 445, 36-42.

Search for more literature for 1.14.12.23

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Diaphorobacter sp. DS2

Protein Variants

Protein Variants	Comment	Organism
additional information	individual expression of each subunit and separate purification. The active recombinant enzyme can be reconstituted in vitro by mixing all three purified subunits	Diaphorobacter sp. DS2

Organism

Organism	UniProt	Comment	Textmining
Diaphorobacter sp. DS2	M9PTH9 and M9PW10 and M9PSS0 and M9PV03	M9PTH9 i.e. ferredoxin reductase subunit MntAa, M9PW10 i.e. oxygenase alpha subunit MntAc, M9PSS0 i.e. ferredoxin subunit MntAb, M9PV03 i.e. oxygenase beta subunit MntAd	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,6-dinitrotoluene + NADH + O2	-	Diaphorobacter sp. DS2	3-methyl-4-nitrocatechol + nitrite + NAD+	84% of the activity with 3-nitrotoluene	?
2-chloronitrobenzene + NADH + O2	-	Diaphorobacter sp. DS2	3-chlorocatechol + nitrite + NAD+	80% of the activity with 3-nitrotoluene	?
2-nitrotoluene + NADH + O2	-	Diaphorobacter sp. DS2	3-methylcatechol + nitrite + NAD+	71% of the activity with 3-nitrotoluene. Products are 91% 3-methylcatechol, 9% 3-nitrobenzyl alcohol	?
3-nitrotoluene + NADH + O2	-	Diaphorobacter sp. DS2	3-methylcatechol + 4-methylcatechol + nitrite + NAD+	products are 54% 3-methylcatechol, 39% 4-methylcatechol, 7% 3-nitrobenzyl alcohol	?
nitrobenzene + NADH + O2	-	Diaphorobacter sp. DS2	catechol + nitrite + NAD+	78% of the activity with 3-nitrotoluene	?

Synonyms

Synonyms	Comment	Organism
MntAa	-	Diaphorobacter sp. DS2
MntAb	-	Diaphorobacter sp. DS2
MntAc	-	Diaphorobacter sp. DS2
MntAd	-	Diaphorobacter sp. DS2

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Release 2023.1 (January 2023)