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Literature summary for 1.14.12.18 extracted from

  • Barriault, D.; Simard, C.; Chatel, H.; Sylvestre, M.
    Characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356 (2001), Can. J. Microbiol., 47, 1025-1032.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras Comamonas testosteroni
additional information characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
strain LB400, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356
-
Comamonas testosteroni
-
strain B-356, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2',5,5'-tetrachlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. ?
-
?
2,2'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. ?
-
?
2,2'-dichlorobiphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
?
3,3'-dichlorobiphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
?
4,4'-dichlorobiphenyl + NADH + H+ + O2
-
Burkholderia sp. ?
-
?
biphenyl + NADH + H+ + O2
-
Burkholderia sp. ?
-
?
biphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
?
additional information no activity with 3,3'-dichlorobiphenyl Burkholderia sp. ?
-
?
additional information no activity with 4,4'-dichlorobiphenyl and 2,2',5,5'-tetrachlorobiphenyl Comamonas testosteroni ?
-
?

Synonyms

Synonyms Comment Organism
BPDO
-
Comamonas testosteroni
BPDO
-
Burkholderia sp.