Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.12.15 extracted from

  • Schlaefli, H.R.; Weiss, M.A.; Leisinger, T.; Cook, A.M.
    Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component (1994), J. Bacteriol., 176, 6644-6652.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme is a Rieske [2Fe-2S] protein Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni
49000
-
2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni
126000
-
oxygenase component of the TER dioxygenase system, gel filtration Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Comamonas testosteroni T-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
oxygenase component of the TER dioxygenase system. The reductase component or components cannot be purified Comamonas testosteroni

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:terephthalate-grown cell
-
Comamonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-Dicarboxynaphthalene + O2
-
Comamonas testosteroni ?
-
?
1,4-Dicarboxynaphthalene + O2
-
Comamonas testosteroni T-2 ?
-
?
2,5-Dicarboxypyridine + O2
-
Comamonas testosteroni ?
-
?
2,5-Dicarboxypyridine + O2
-
Comamonas testosteroni T-2 ?
-
?
terephthalate + NADH + O2 + H+
-
Comamonas testosteroni (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
?
terephthalate + NADH + O2 + H+
-
Comamonas testosteroni T-2 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 36 oxygenase component of the TER dioxygenase system Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 oxygenase component of the TER dioxygenase system Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NADH
-
Comamonas testosteroni