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Literature summary for 1.14.12.13 extracted from

  • Francisco, P.B.; Ogawa, N.; Suzuki, K.; Miyashita, K.
    The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates (2001), Microbiology, 147, 121-133.
    View publication on PubMed
Search for more literature for 1.14.12.13

Cloned(Commentary)

Cloned (Comment) Organism
several gene constructs are expressed in Escherichia coli DH5alpha, S17-1, S17-lambdapir and HMS174(DE3) Burkholderia sp.

Protein Variants

Protein Variants Comment Organism
additional information disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8 Burkholderia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Burkholderia sp.
Sulfide
-
 Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chlorobenzoate + NADH + H+ + O2 Burkholderia sp.
-
 catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2 Burkholderia sp. NK8
-
 catechol + chloride + NAD+ + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
 isolated from soil
-
Burkholderia sp. NK8
-
 isolated from soil
-

Reaction

Reaction Comment Organism Reaction ID
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 broad substrate specificity, ability to transform all 3 monochlorobenzoates almost equally well. Enzyme appears to lack absolute regioselectivity Burkholderia sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
 cells are grown on 3-chlorobenzoate, benzoate as substrate for assay Burkholderia sp.
0.0024
-
 recombinant cells are grown on LB/IPTG, benzoate as substrate for assay Burkholderia sp.
0.0032
-
 cells are grown on benzoate, benzoate as substrate for assay Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2 no growth on 2-chlorobenzoate Burkholderia sp. catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. NK8 catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2 no growth on 2-chlorobenzoate Burkholderia sp. NK8 catechol + chloride + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. 3-chlorocatechol + NAD+ + CO2
-
 ?
2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. NK8 3-chlorocatechol + NAD+ + CO2
-
 ?
3-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. 4-chlorocatechol + NAD+ + CO2
-
 ?
3-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. NK8 4-chlorocatechol + NAD+ + CO2
-
 ?
3-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. 3-chlorocatechol + NAD+ + CO2
-
 ?
4-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. 4-chlorocatechol + NAD+ + CO2
-
 ?
benzoate + NADH + H+ + O2 2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate Burkholderia sp. catechol + NAD+ + CO2
-
 ?
benzoate + NADH + H+ + O2 2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate Burkholderia sp. NK8 catechol + NAD+ + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Burkholderia sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 assay at Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Burkholderia sp.
NADH
-
 Burkholderia sp.