Literature summary for 1.14.12.11 extracted from

Lee, K.; Friemann, R.; Parales, J.V.; Gibson, D.T.; Ramaswamy, S.
Purification, crystallization and preliminary X-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system (2005), Acta crystallogr. Sect. F, 61, 669-672.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain CGSC7692 (pDTG601A)	Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop and sitting drop vapour diffusion method using 40% (w/v) polyethylene glycol 600, 0.1 M sodium citrate pH 6.1 and 20-50 mM Fe(NH4)2(SO4)	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose FF column chromatography	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
toluene + NADH + O2	-	Pseudomonas putida	(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
oxygenaseTOL	-	Pseudomonas putida
TDO	-	Pseudomonas putida
toluene 2,3-dioxygenase	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas putida

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Release 2023.1 (January 2023)