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Literature summary for 1.14.12.11 extracted from

  • Subramanian, V.; Liu, T.N.; Yeh, W.K.; Narro, M.; Gibson, D.T.
    Purification and properties of NADH-ferredoxinTOL reductase. A component of toluene dioxygenase from Pseudomonas putida (1981), J. Biol. Chem., 256, 2723-2730.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of the NADH-ferredoxinTOL reductase component: 0.0046 mM for cytochrome and 0.0105 mM for NADH Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida
46500
-
 NADH-ferredoxinTOL reductase component, gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NADH-ferredoxinTOL reductase component of toluene dioxygenase Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, purified NADH-ferredoxinTOL reductase component is stable for two weeks Pseudomonas putida
0-4°C, purified NADH-ferredoxinTOL reductase component is stable for up to 30 h Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the NADH-ferredoxinTOL reductase component catalyzes the NADH-dependent reduction of 2,6-dichloroindophenol, nitroblue tetrazolium and ferricyanide. NADPH is inactive Pseudomonas putida ?
-
 ?
toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.5 NADH-ferredoxinTOL reductase component Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD NADH-ferredoxinTOL reductase component is a flavoprotein that contains one mol of FAD per mol of enzyme. Km: 2.5 nM Pseudomonas putida
NADH NADH-ferredoxinTOL reductase component Pseudomonas putida
NADPH NADPH is inactive Pseudomonas putida