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Literature summary for 1.14.12.10 extracted from

  • Yamaguchi, M.; Fujisawa, H.
    Purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1 (1980), J. Biol. Chem., 255, 5058-5063.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
benzoate
-
Pseudomonas putida
0.0043
-
O2
-
Pseudomonas putida
0.0048
-
NADH
-
Pseudomonas putida
0.026
-
NADH-cytochrome c reductase component
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 10 mol of iron per mol of enzyme, [2Fe-2S] clusters Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
NADH-cytochrome c component Pseudomonas putida
270000
-
oxygenase component, gel filtration Pseudomonas putida
273000
-
oxygenase component, sedimentation equilibrium Pseudomonas putida
280000
-
oxygenase component, native PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
C-1
-
Pseudomonas putida C-1
-
C-1
-

Purification (Commentary)

Purification (Comment) Organism
oxygenase component Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ the enzyme consists of two proteins, a NADH-cytochrome c reductase and an oxygenase Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminobenzoate + O2 + NADH
-
Pseudomonas putida 3-amino-2-hydro-1,2-dihydroxybenzoate
-
?
3-aminobenzoate + O2 + NADH
-
Pseudomonas putida C-1 3-amino-2-hydro-1,2-dihydroxybenzoate
-
?
3-chlorobenzoate + O2 + NADH + H+
-
Pseudomonas putida 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-chlorobenzoate + O2 + NADH + H+
-
Pseudomonas putida C-1 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-fluorobenzoate + O2 + NADH + H+ benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution Pseudomonas putida 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-fluorobenzoate + O2 + NADH + H+ benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution Pseudomonas putida C-1 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-methylbenzoate + O2 + NADH + H+
-
Pseudomonas putida 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
4-fluorobenzoate + O2 + NADH
-
Pseudomonas putida 4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
?
4-fluorobenzoate + O2 + NADH
-
Pseudomonas putida C-1 4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
?
benzoate + NADH-cytochrome c reductase component + H+ + O2
-
Pseudomonas putida (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+-cytochrome c reductase component
-
r
benzoate + O2 + NADH + H+
-
Pseudomonas putida 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH + H+
-
Pseudomonas putida C-1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
367
-
benzoate in the presence of saturating amounts of NADH-cytochrome c reductase Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
oxygenase component Pseudomonas putida
8.2
-
NADH-cytochrome c reductase component Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 55% activity, pH 8.0: about 60% activity Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters Pseudomonas putida
NADH requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters Pseudomonas putida