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Literature summary for 1.14.11.B16 extracted from

  • Fraser, P.D.; Miura, Y.; Misawa, N.
    In vitro characterization of astaxanthin biosynthetic enzymes (1997), J. Biol. Chem., 272, 6128-6135.
    View publication on PubMed
Search for more literature for 1.14.11.B16

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate activation Alcaligenes sp.
2-oxoglutarate activation Haematococcus lacustris
2-oxoglutarate activation Agrobacterium aurantiacum
ascorbate activation Alcaligenes sp.
ascorbate activation Haematococcus lacustris
ascorbate activation Agrobacterium aurantiacum
catalase activation Alcaligenes sp.
catalase activation Haematococcus lacustris
catalase activation Agrobacterium aurantiacum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Alcaligenes sp.
expressed in Escherichia coli Haematococcus lacustris
expressed in Escherichia coli Agrobacterium aurantiacum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activation Alcaligenes sp.
Fe2+ activation Haematococcus lacustris
Fe2+ activation Agrobacterium aurantiacum

Organism

Organism UniProt Comment Textmining
Agrobacterium aurantiacum
-
-
-
Alcaligenes sp.
-
-
-
Haematococcus lacustris
-
-
-

Reaction

Reaction Comment Organism Reaction ID
beta-carotene + 2-oxoglutarate + O2 + H2O = canthaxanthin + succinate + CO2 + 4 H+ echinenone is an intermediary product Alcaligenes sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000083
-
 substrate beta-carotene, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Haematococcus lacustris
0.000000083
-
 substrate beta-carotene, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Agrobacterium aurantiacum
0.000000083
-
 substrate zeaxanthin, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Alcaligenes sp.
0.00000015
-
 substrate beta-carotene, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Alcaligenes sp.
0.00000017
-
 substrate zeaxanthin, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Haematococcus lacustris
0.0000002
-
 substrate zeaxanthin, 30°C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid Agrobacterium aurantiacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 2-oxoglutarate + O2
-
 Alcaligenes sp. canthaxanthin + ?
-
 ?
beta-carotene + 2-oxoglutarate + O2
-
 Haematococcus lacustris canthaxanthin + ?
-
 ?
beta-carotene + 2-oxoglutarate + O2
-
 Agrobacterium aurantiacum canthaxanthin + ?
-
 ?
zeaxanthin + 2-oxoglutarate + O2
-
 Alcaligenes sp. 4-oxozeaxanthin + ?
-
 ?
zeaxanthin + 2-oxoglutarate + O2
-
 Haematococcus lacustris 4-oxozeaxanthin + ?
-
 ?
zeaxanthin + 2-oxoglutarate + O2
-
 Agrobacterium aurantiacum 4-oxozeaxanthin + ?
-
 ?

Synonyms

Synonyms Comment Organism
4,(4')-beta-ionone ring oxygenase
-
 Alcaligenes sp.
4,(4')-beta-ionone ring oxygenase
-
 Haematococcus lacustris
4,(4')-beta-ionone ring oxygenase
-
 Agrobacterium aurantiacum
CrtW
-
 Alcaligenes sp.
CrtW
-
 Haematococcus lacustris
CrtW
-
 Agrobacterium aurantiacum

Cofactor

Cofactor Comment Organism Structure
additional information no activation by NAD*, NADP+, NADPH, FAD, FMN Haematococcus lacustris
additional information no activation by NAD+, NADP+, NADPH, FAD, FMN Alcaligenes sp.
additional information no activation by NAD+, NADP+, NADPH, FAD, FMN Agrobacterium aurantiacum