Cloned (Comment) | Organism |
---|---|
gene F3H1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses | Reaumuria trigyna |
gene F3H2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses | Reaumuria trigyna |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Reaumuria trigyna | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Reaumuria trigyna | W0S9B2 | collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China | - |
Reaumuria trigyna | W0SDE6 | collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | high transcript levels of gene RtF3H2 in roots and stems | Reaumuria trigyna | - |
seedling | - |
Reaumuria trigyna | - |
stem | high transcript levels of gene RtF3H1 in stems, high transcript levels of gene RtF3H2 in roots and stems | Reaumuria trigyna | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Reaumuria trigyna | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39010, sequence calculation | Reaumuria trigyna |
? | x * 39520, sequence calculation | Reaumuria trigyna |
Synonyms | Comment | Organism |
---|---|---|
F3H1 | - |
Reaumuria trigyna |
F3H2 | - |
Reaumuria trigyna |
flavanone-3-hydroxylase | - |
Reaumuria trigyna |
RtF3H1 | - |
Reaumuria trigyna |
RtF3H2 | - |
Reaumuria trigyna |
Organism | Comment | Expression |
---|---|---|
Reaumuria trigyna | transcript levels of the two F3H genes, RtF3H1 and RtF3H2, are increased in Reaumuria trigyna not only under salt stress but also under drought and cold stresses, and by abscisic acid | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family | Reaumuria trigyna |
additional information | the 2S-naringenin substrate binding site of RtF3H1 consists of Ser135, Try149, Val214, Arg299, and Val304 | Reaumuria trigyna |
additional information | the 2S-naringenin substrate binding site of RtF3H2 consists of Ser135, Phe149, Ile214, Arg299, and Ala305 | Reaumuria trigyna |
physiological function | the F3H gene, which encodes flavanone-3-hydroxylase, is an essential gene in the flavonoid biosynthetic pathway | Reaumuria trigyna |