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Literature summary for 1.14.11.9 extracted from

  • Halbwirth, H.; Fischer, T.C.; Schlangen, K.; Rademacher, W.; Schleifer, K.; Forkmann, G.; Stich, K.
    Screening for inhibitors of 2-oxoglutarate-dependent dioxygenases: Flavanone 3?-hydroxylase and flavonol synthase (2006), Plant Sci., 171, 194-205.
    View publication on PubMed
Search for more literature for 1.14.11.9

Activating Compound

Activating Compound Comment Organism Structure
piperazine-1,4-bis-(2-ethane sulfonic acid) 1 mM, 96% relative activity Pyrus communis
piperazine-1,4-bis-(2-ethane sulphonic acid) 1 mM, 96% relative activity Malus domestica
pyridine-2,3-dicarboxylic acid 1 mM 205% relative activity, 0.1 mM 120% relative activity Malus domestica
pyridine-2,3-dicarboxylic acid 1 mM 205% relative activity, 0.1 mM 120% relative activity Pyrus communis
pyridine-2,6-dicarboxylic acid 1 mM 220% relative activity, 0.1 mM 186% relative activity Malus domestica
pyridine-2,6-dicarboxylic acid 1 mM 220% relative activity, 0.1 mM 186% relative activity Pyrus communis
pyridine-2,6-dicarboxylic acid chloride 1 mM, 196% relative activity, 0.1 mM 194% relative activity Malus domestica
pyridine-2,6-dicarboxylic acid chloride 1 mM, 196% relative activity, 0.1 mM 194% relative activity Pyrus communis
pyrrole-3,4-dicarboxylic acid diethyl ester 1 mM, 113% relative activity Malus domestica
pyrrole-3,4-dicarboxylic acid diethyl ester 1 mM, 113% relative activity Pyrus communis
sodium 4,6-dioxo-2,2-dimethyl-5-(1-alloxyamino-butylidene)-cyclohexane-1-carboxylic acid methyl ester 0.1 mM 115% relative activity Malus domestica
sodium 4,6-dioxo-2,2-dimethyl-5-(1-alloxyamino-butylidene)-cyclohexane-1-carboxylic acid methyl ester 0.1 mM 115% relative activity Pyrus communis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast strain InvSc1 Malus domestica
expressed in yeast strain InvSc1 Pyrus communis

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxy-5-oxo-4-butyryl-cyclohex-3-ene-1-carboxylic acid ethyl ester 1 mM 36% relative activity, 0.1 mM 68% relative activity Malus domestica
3-hydroxy-5-oxo-4-butyryl-cyclohex-3-ene-1-carboxylic acid ethyl ester 1 mM 36% relative activity, 0.1 mM 68% relative activity Pyrus communis
3-hydroxy-5-oxo-4-cyclopropanecarbonyl-cyclohex-3-ene-1-carboxylic acid ethyl ester 1 mM 9% relative activity, 0.1 mM 38% relative activity Malus domestica
3-hydroxy-5-oxo-4-cyclopropanecarbonyl-cyclohex-3-ene-1-carboxylic acid ethyl ester 1 mM 9% relative activity, 0.1 mM 38% relative activity Pyrus communis
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-(2-dimethylamino)-thiazole 1 mM 25% relative activity, 0.1 mM 56% relative activity Malus domestica
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-(2-dimethylamino)-thiazole 1 mM 25% relative activity, 0.1 mM 56% relative activity Pyrus communis
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carbaldehyde 1 mM 33% relative activity, 0.1 mM 75% relative activity Malus domestica
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carbaldehyde 1 mM 33% relative activity, 0.1 mM 75% relative activity Pyrus communis
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carbothioic acid S-ethyl ester 1 mM 15% relative activity, 0.1 mM 40% relative activity Malus domestica
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carbothioic acid S-ethyl ester 1 mM 15% relative activity, 0.1 mM 40% relative activity Pyrus communis
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-pentanoic acid 1 mM 13% relative activity, 0.1 mM 75% relative activity Malus domestica
3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-pentanoic acid 1 mM 13% relative activity, 0.1 mM 75% relative activity Pyrus communis
benzene-1,2,4,5-tetracarboxylic acid 1 mM 95% relative activity Malus domestica
benzene-1,2,4,5-tetracarboxylic acid 1 mM 95% relative activity Pyrus communis
calcium 3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carboxylate 1 mM 10% relative activity, 0.1 mM 33% relative activity Malus domestica
calcium 3-hydroxy-5-oxo-4-propionyl-cyclohex-3-ene-1-carboxylate 1 mM 10% relative activity, 0.1 mM 33% relative activity Pyrus communis
pyridine-2,4-dicarboxylic acid diethyl ester 1 mM 7% relative activity, 0.1 mM 21% relative activity Malus domestica
pyridine-2,4-dicarboxylic acid diethyl ester 1 mM 7% relative activity, 0.1 mM 21% relative activity Pyrus communis
pyridine-2,5-dicarboxylic acid 1 mM 3% relative activity, 0.1 mM 11% relative activity Malus domestica
pyridine-2,5-dicarboxylic acid 1 mM 3% relative activity, 0.1 mM 11% relative activity Pyrus communis
sodium 4,6-dioxo-2,2-dimethyl-5-(1-alloxyamino-butylidene)-cyclohexane-1-carboxylic acid methyl ester 1 mM 89% relative activity Malus domestica
sodium 4,6-dioxo-2,2-dimethyl-5-(1-alloxyamino-butylidene)-cyclohexane-1-carboxylic acid methyl ester 1 mM 89% relative activity Pyrus communis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Malus domestica
Fe2+ required Pyrus communis

Organism

Organism UniProt Comment Textmining
Malus domestica
-
 cultivars M9 and Weirouge
-
Pyrus communis
-
 cultivars Pyrodwarf and Conference
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Malus domestica
-
leaf
-
 Pyrus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eriodictyol + 2-oxoglutarate + O2
-
 Malus domestica (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
eriodictyol + 2-oxoglutarate + O2
-
 Pyrus communis (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Malus domestica (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Pyrus communis (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
F3H
-
 Malus domestica
F3H
-
 Pyrus communis
FHT
-
 Malus domestica
FHT
-
 Pyrus communis
flavanone 3beta-hydroxylase
-
 Malus domestica
flavanone 3beta-hydroxylase
-
 Pyrus communis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Malus domestica
30
-
-
 Pyrus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.25
-
-
 Malus domestica
7.25
-
-
 Pyrus communis

Cofactor

Cofactor Comment Organism Structure
ascorbate required Malus domestica
ascorbate required Pyrus communis