Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | sparing as well as augmenting effect | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-N-Acetyltrimethyllysine | - |
Bos taurus | |
citrate | - |
Bos taurus | |
Hydroxytrimethyllysine | 4 stereoisomers | Bos taurus | |
isocitrate | - |
Bos taurus | |
Lactate | - |
Bos taurus | |
malonate | - |
Bos taurus | |
p-chloromercuribenzoate | - |
Bos taurus | |
succinate | - |
Bos taurus | |
Zn2+ | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
N6,N6,N6-Trimethyl-L-lysine | - |
Bos taurus | |
0.22 | - |
alpha-ketoglutarate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no stimulation | Bos taurus | |
Fe2+ | required | Bos taurus | |
Fe2+ | preincubation with Fe2+ in absence of other cofactors inactivates (ascorbate or dithiothreitol prevents inactivation) | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000 + x * 65000, SDS-PAGE | Bos taurus |
65000 | - |
x * 52000 + x * 65000, SDS-PAGE | Bos taurus |
180000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | cortex (67%), medulla (33%) | Bos taurus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
0°C, half-life: 5 days | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine | Bos taurus | 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000 + x * 65000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
half-life: 5 days | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Bos taurus |