Literature summary for 1.14.11.53 extracted from

Zou, S.; Toh, J.D.; Wong, K.H.; Gao, Y.G.; Hong, W.; Woon, E.C.
N6-methyladenosine: a conformational marker that regulates the substrate specificity of human demethylases FTO and ALKBH5 (2016), Sci. Rep., 6, 25677.

Search for more literature for 1.14.11.53

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Rosetta cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.755	-	GCGG(m6A)CUCCAGAUG	at pH 7.4 and 37°C	Homo sapiens
2.251	-	GA(m6A)CA	at pH 7.4 and 37°C	Homo sapiens
2.334	-	GG(m6A)CU	at pH 7.4 and 37°C	Homo sapiens
2.583	-	CCCC(m6A)CCCCCCCCC	at pH 7.4 and 37°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N6-methyladenine in mRNA + 2-oxoglutarate + O2	Homo sapiens	-	adenine in mRNA + formaldehyde + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni affinity column chromatography, HiTrap Q column chromatography, and Superdex 75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CCCC(m6A)CCCCCCCCC + 2-oxoglutarate + O2	30% demethylation	Homo sapiens	? + formaldehyde + succinate + CO2	-	?
GA(m6A)CA + 2-oxoglutarate + O2	39% demethylation	Homo sapiens	GAACA + formaldehyde + succinate + CO2	-	?
GCGG(m6A)CUCCAGAUG + 2-oxoglutarate + O2	31% demethylation	Homo sapiens	GCGGACUCCAGAUG + formaldehyde + succinate + CO2	-	?
GG(m6A)CU + 2-oxoglutarate + O2	37% demethylation	Homo sapiens	GGACU + formaldehyde + succinate + CO2	-	?
N6-methyladenine in mRNA + 2-oxoglutarate + O2	-	Homo sapiens	adenine in mRNA + formaldehyde + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
ALKBH5	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0023	-	GG(m6A)CU	at pH 7.4 and 37°C	Homo sapiens
0.0023	-	CCCC(m6A)CCCCCCCCC	at pH 7.4 and 37°C	Homo sapiens
0.0027	-	GA(m6A)CA	at pH 7.4 and 37°C	Homo sapiens
0.0029	-	GCGG(m6A)CUCCAGAUG	at pH 7.4 and 37°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.88	-	CCCC(m6A)CCCCCCCCC	at pH 7.4 and 37°C	Homo sapiens
1	-	GG(m6A)CU	at pH 7.4 and 37°C	Homo sapiens
1.2	-	GA(m6A)CA	at pH 7.4 and 37°C	Homo sapiens
1.6	-	GCGG(m6A)CUCCAGAUG	at pH 7.4 and 37°C	Homo sapiens

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Release 2023.1 (January 2023)