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Literature summary for 1.14.11.53 extracted from

  • Jia, G.; Yang, C.G.; Yang, S.; Jian, X.; Yi, C.; Zhou, Z.; He, C.
    Oxidative demethylation of 3-methylthymine and 3-methyluracil in single-stranded DNA and RNA by mouse and human FTO (2008), FEBS Lett., 582, 3313-3319.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Star (DE3) Homo sapiens
expression in Escherichia coli BL21 Star (DE3) Mus musculus

General Stability

General Stability Organism
the purified recombinant mRNA N6-methyladenine demethylase appears to be more stable than the purified recombinant murine mRNA N6-methyladenine demethylase in vitro Homo sapiens
the purified recombinant mRNA N6-methyladenine demethylase appears to be more stable than the purified recombinant murine mRNA N6-methyladenine demethylase in vitro Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate at millimolar concentrations Homo sapiens
2-oxoglutarate at millimolar concentrations Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00095
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Homo sapiens
0.00192
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Mus musculus
0.00208
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Mus musculus
0.00851
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains Fe2+ Homo sapiens
Fe2+ contains Fe2+ Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-methyladenine in mRNA + 2-oxoglutarate + O2 Homo sapiens
-
 adenine in mRNA + formaldehyde + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9C0B1
-
-
Mus musculus Q8BGW1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information very low activity toward repairing N1-methyladenine in 49 mer single-stranded DNA Homo sapiens ?
-
 ?
additional information very low activity toward repairing N1-methyladenine in 49 mer single-stranded DNA Mus musculus ?
-
 ?
N3-methylcytosine in single-stranded DNA + 2-oxoglutarate + O2 strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA Homo sapiens cytosine in single-stranded DNA + formaldehyde + succinate + CO2
-
 ?
N3-methylcytosine in single-stranded DNA + 2-oxoglutarate + O2 strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA Mus musculus cytosine in single-stranded DNA + formaldehyde + succinate + CO2
-
 ?
N3-methylthymine in single-stranded DNA + 2-oxoglutarate + O2 strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA. Negligible activities against N3-methylthymine in double-stranded DNA Homo sapiens thymine in single-stranded DNA + formaldehyde + succinate + CO2
-
 ?
N3-methylthymine in single-stranded DNA + 2-oxoglutarate + O2 strong preference toward N3-methylthymine over N3-methylcytosine in single-stranded DNA. Negligible activities against N3-methylthymine in double-stranded DNA Mus musculus thymine in single-stranded DNA + formaldehyde + succinate + CO2
-
 ?
N3-methyluracil in single-stranded mRNA + 2-oxoglutarate + O2 2-fold preference for N3-methyluracil in single-stranded mRNA as the substrate over N3-methylthymine in single-stranded DNA. Slightly higher efficiency over that of N3-methylthymine in single-stranded DNA Homo sapiens uracil in single-stranded mRNA + formaldehyde + succinate + CO2
-
 ?
N3-methyluracil in single-stranded mRNA + 2-oxoglutarate + O2 2-fold preference for N3-methyluracil in single-stranded mRNA as the substrate over N3-methylthymine in single-stranded DNA. Slightly higher efficiency over that of N3-methylthymine in single-stranded DNA Mus musculus uracil in single-stranded mRNA + formaldehyde + succinate + CO2
-
 ?
N6-methyladenine in mRNA + 2-oxoglutarate + O2
-
 Homo sapiens adenine in mRNA + formaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
FTO
-
 Homo sapiens
FTO
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Homo sapiens
20
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000117
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Homo sapiens
0.00148
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Mus musculus
0.0019
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Homo sapiens
0.0033
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Mus musculus
6
-
 demethylation of N3-methylthymine in single-stranded DNA and N3-methyluracil in single-stranded RNA Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.223
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Homo sapiens
0.77
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Mus musculus
1.23
-
 N3-methylthymine in single-stranded DNA pH 6.0, 20°C Homo sapiens
1.58
-
 N3-methyluracil in single-stranded mRNA pH 6.0, 20°C Mus musculus