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Literature summary for 1.14.11.49 extracted from

  • Yang, Z.; Unrine, J.; Nonaka, K.; Van Lanen, S.G.
    Fe(II)-dependent, uridine-5-monophosphate alpha-ketoglutarate dioxygenases in the synthesis of 5'-modified nucleosides (2012), Methods Enzymol., 516, 153-168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38200
-
 x * 38200, calculated from amino acid sequence Streptomyces sp.
40000
-
 x * 40000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UMP + 2-oxoglutarate + O2 Streptomyces sp.
-
 5'-dehydrouridine + succinate + CO2 + phosphate
-
 ?
UMP + 2-oxoglutarate + O2 Streptomyces sp. SANK 60405
-
 5'-dehydrouridine + succinate + CO2 + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. SANK 60405
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UMP + 2-oxoglutarate + O2
-
 Streptomyces sp. 5'-dehydrouridine + succinate + CO2 + phosphate
-
 ?
UMP + 2-oxoglutarate + O2
-
 Streptomyces sp. SANK 60405 5'-dehydrouridine + succinate + CO2 + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Streptomyces sp.
? x * 38200, calculated from amino acid sequence Streptomyces sp.

Synonyms

Synonyms Comment Organism
LIPL
-
 Streptomyces sp.
UMP:alpha-ketoglutarate dioxygenase
-
 Streptomyces sp.
uridine-5-monophosphate alpha-ketoglutarate dioxygenase
-
 Streptomyces sp.