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Literature summary for 1.14.11.45 extracted from

  • Smirnov, S.V.; Sokolov, P.M.; Kodera, T.; Sugiyama, M.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
    A novel family of bacterial dioxygenases that catalyse the hydroxylation of free L-amino acids (2012), FEMS Microbiol. Lett., 331, 97-104.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
L-isoleucine Km below 0.3 mM, at pH 7.0 and 34°C Bacillus thuringiensis
2.3
-
L-leucine at pH 7.0 and 34°C Bacillus thuringiensis
3.2
-
L-methionine at pH 7.0 and 34°C Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis
-
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis 2-e-2
-
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis E2GIN1
-
-
Bacillus thuringiensis 2-e-2 E2GIN1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?
L-isoleucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis 2-e-2 (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?
L-leucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis ?
-
?
L-leucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis 2-e-2 ?
-
?
L-methionine + 2-oxoglutarate + O2
-
Bacillus thuringiensis ?
-
?
L-methionine + 2-oxoglutarate + O2
-
Bacillus thuringiensis 2-e-2 ?
-
?
additional information no activity with L-threonine Bacillus thuringiensis ?
-
?
additional information no activity with L-threonine Bacillus thuringiensis 2-e-2 ?
-
?

Synonyms

Synonyms Comment Organism
IDO
-
Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
L-isoleucine at pH 7.0 and 34°C Bacillus thuringiensis
7.5
-
L-leucine at pH 7.0 and 34°C Bacillus thuringiensis
33
-
L-methionine at pH 7.0 and 34°C Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2
-
L-leucine at pH 7.0 and 34°C Bacillus thuringiensis
10
-
L-methionine at pH 7.0 and 34°C Bacillus thuringiensis
19
-
L-isoleucine kcat/Km above 19 1/sec*mM, at pH 7.0 and 34°C Bacillus thuringiensis