Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-isoleucine | Km below 0.3 mM, at pH 7.0 and 34°C | Bacillus thuringiensis | |
2.3 | - |
L-leucine | at pH 7.0 and 34°C | Bacillus thuringiensis | |
3.2 | - |
L-methionine | at pH 7.0 and 34°C | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis 2-e-2 | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | E2GIN1 | - |
- |
Bacillus thuringiensis 2-e-2 | E2GIN1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2-e-2 | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-leucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
L-leucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2-e-2 | ? | - |
? | |
L-methionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
L-methionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2-e-2 | ? | - |
? | |
additional information | no activity with L-threonine | Bacillus thuringiensis | ? | - |
? | |
additional information | no activity with L-threonine | Bacillus thuringiensis 2-e-2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
L-isoleucine | at pH 7.0 and 34°C | Bacillus thuringiensis | |
7.5 | - |
L-leucine | at pH 7.0 and 34°C | Bacillus thuringiensis | |
33 | - |
L-methionine | at pH 7.0 and 34°C | Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
L-leucine | at pH 7.0 and 34°C | Bacillus thuringiensis | |
10 | - |
L-methionine | at pH 7.0 and 34°C | Bacillus thuringiensis | |
19 | - |
L-isoleucine | kcat/Km above 19 1/sec*mM, at pH 7.0 and 34°C | Bacillus thuringiensis |