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Literature summary for 1.14.11.45 extracted from

  • Kodera, T.; Smirnov, S.; Samsonova, N.; Kozlov, Y.; Koyama, R.; Hibi, M.; Ogawa, J.; Yokozeki, K.; Shimizu, S.
    A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine (2009), Biochem. Biophys. Res. Commun., 390, 506-510.
    View publication on PubMed
Search for more literature for 1.14.11.45

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid ascorbic acid is not necessarily required for the reaction, however, in the presence of ascorbic acid, the reaction rate is greatly stimulated Bacillus thuringiensis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 1 mM Bacillus thuringiensis
EDTA complete inhibition at 1 mM Bacillus thuringiensis
Zn2+ complete inhibition at 1 mM Bacillus thuringiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Bacillus thuringiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis
-
 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis 2e2 AKU 0251
-
 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
-
-
Bacillus thuringiensis 2e2 AKU 0251
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiPrep DEAE column chromatography, Mono S column chromatography, Resource PHE column chromatography, and Superdex 75 gel filtration Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?
L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis 2e2 AKU 0251 (2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Bacillus thuringiensis

Synonyms

Synonyms Comment Organism
IDO
-
 Bacillus thuringiensis
L-isoleucine dioxygenase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus thuringiensis