Protein Variants | Comment | Organism |
---|---|---|
F171A | exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 245% compared to the wild-type value | Sphingobium herbicidovorans |
F171Q | exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 70% compared to the wild-type value | Sphingobium herbicidovorans |
I106A | exhibits about 30% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 5% compared to the wild-type value | Sphingobium herbicidovorans |
I106G/G107I | exhibits 0.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
I106G/G107N | exhibits 8.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
L83A | exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
Q93A | exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 14% compared to the wild-type value | Sphingobium herbicidovorans |
R285A | exhibits 14.8% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 5% compared to the wild-type value | Sphingobium herbicidovorans |
R285A | exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
V80A | exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 17% compared to the wild-type value | Sphingobium herbicidovorans |
Y221A | exhibits 5.2% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
Y221A | exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-2-(4-chloro-2-methylphenoxy)propanoate | Q93A variant retains 60% of wild-type enzyme activity, but this drops to 35% when the racemate is provided, suggesting that Gln93 prevents the incorrect enantiomer from binding to and inhibiting the enzyme | Sphingobium herbicidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171A | Sphingobium herbicidovorans | |
0.38 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans | |
0.438 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171Q | Sphingobium herbicidovorans | |
1.55 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R285A | Sphingobium herbicidovorans | |
2.3 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme V80A | Sphingobium herbicidovorans | |
2.8 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme Q93A | Sphingobium herbicidovorans | |
8.9 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme I106A | Sphingobium herbicidovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium herbicidovorans | Q8KSC8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium herbicidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (R)-dichlorprop. RdpA exclusively transforms the (R)-enantiomer of dichlorprop | Sphingobium herbicidovorans | 2,4-dichlorophenol + pyruvate + succinate + CO2 | - |
? | |
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (R)-mecoprop. RdpA exclusively transforms the (R)-enantiomer of mecoprop | Sphingobium herbicidovorans | 4-chloro-2-methylphenol + pyruvate + succinate + CO2 | - |
? | |
additional information | construction of homology models of SdpA and RdpA from Sphingomonas herbicidovorans MH and the use of docking to identify residues likely to be involved in herbicide binding | Sphingobium herbicidovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RdpA | - |
Sphingobium herbicidovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sphingobium herbicidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R285A | Sphingobium herbicidovorans | |
2.9 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171A | Sphingobium herbicidovorans | |
3.38 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171Q | Sphingobium herbicidovorans | |
4 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme I106A | Sphingobium herbicidovorans | |
4.13 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme V80A | Sphingobium herbicidovorans | |
4.2 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans | |
4.33 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme Q93A | Sphingobium herbicidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Sphingobium herbicidovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme I106A | Sphingobium herbicidovorans | |
0.56 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R285A | Sphingobium herbicidovorans | |
1.55 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme Q93A | Sphingobium herbicidovorans | |
1.8 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme V80A | Sphingobium herbicidovorans | |
7.7 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171Q | Sphingobium herbicidovorans | |
11 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans | |
26.9 | - |
(R)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme F171A | Sphingobium herbicidovorans |