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Literature summary for 1.14.11.44 extracted from

  • Westendorf, A.; Benndorf, D.; Müller, R.H.; Babel, W.
    The two enantiospecific dichlorprop/alpha-ketoglutarate-dioxygenases from Delftia acidovorans MC1 - protein and sequence data of RdpA and SdpA (2002), Microbiol. Res., 157, 317-322.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
3 * 36000, SDs-PAGE Delftia acidovorans
108000
-
gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 Delftia acidovorans i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 Delftia acidovorans MC1 i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 Delftia acidovorans i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 Delftia acidovorans MC1 i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans P83310
-
-
Delftia acidovorans MC1 P83310
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop Delftia acidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop Delftia acidovorans MC1 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop Delftia acidovorans 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop Delftia acidovorans MC1 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?

Subunits

Subunits Comment Organism
trimer 3 * 36000, SDs-PAGE Delftia acidovorans

Synonyms

Synonyms Comment Organism
(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase
-
Delftia acidovorans
RdpA
-
Delftia acidovorans

pI Value

Organism Comment pI Value Maximum pI Value
Delftia acidovorans isoelectric focusing 5.9 5.8