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Literature summary for 1.14.11.44 extracted from

  • Müller, T.A.; Fleischmann, T.; van der Meer, J.R.; Kohler, H.P.
    Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH (2006), Appl. Environ. Microbiol., 72, 4853-4861.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ascorbate slightly reduced activity in the absence of ascorbate Sphingobium herbicidovorans

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS) Sphingobium herbicidovorans

General Stability

General Stability Organism
inactivated by several freezing and thawing cycles Sphingobium herbicidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
2-oxoglutarate pH 6.8, 30°C Sphingobium herbicidovorans
0.099
-
(R)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C Sphingobium herbicidovorans
0.164
-
(R)-(2,4-dichlorophenoxy)propanoate pH 6.8, 30°C Sphingobium herbicidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ferrous iron is necessary for activity of the enzyme, and other divalent cations could not replace it Sphingobium herbicidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34900
-
3 * 34900, calculated from sequence Sphingobium herbicidovorans
36000
-
3 * 36000, SDs-PAGE Sphingobium herbicidovorans
104000
-
gel filtration Sphingobium herbicidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 Sphingobium herbicidovorans i.e. (R)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 Sphingobium herbicidovorans i.e. (R)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Sphingobium herbicidovorans Q8KSC8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingobium herbicidovorans

Storage Stability

Storage Stability Organism
4°C, stable for up to 8 weeks Sphingobium herbicidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH Sphingobium herbicidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-dichlorprop. RdpA exclusively transforms the (R)-enantiomer of dichlorprop Sphingobium herbicidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH Sphingobium herbicidovorans 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (R)-mecoprop. RdpA exclusively transforms the (R)-enantiomer of mecoprop. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate Sphingobium herbicidovorans 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
?

Subunits

Subunits Comment Organism
trimer 3 * 36000, SDs-PAGE Sphingobium herbicidovorans
trimer 3 * 34900, calculated from sequence Sphingobium herbicidovorans

Synonyms

Synonyms Comment Organism
2-oxoglutarate-dependent (R)-dichlorprop dioxygenase
-
Sphingobium herbicidovorans
alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase
-
Sphingobium herbicidovorans
RdpA
-
Sphingobium herbicidovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 55 stable Sphingobium herbicidovorans
22
-
stable for up to 7 days Sphingobium herbicidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Sphingobium herbicidovorans

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 pH 6.0: about 60% of maximal activity, pH 7.5: about 55% of maximal activity Sphingobium herbicidovorans

General Information

General Information Comment Organism
physiological function the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH Sphingobium herbicidovorans