Protein Variants | Comment | Organism |
---|---|---|
E69A | exhibits 30.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 42% compared to the wild-type value | Sphingobium herbicidovorans |
G97I/N98G | exhibits 3.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.6% compared to the wild-type value | Sphingobium herbicidovorans |
G97N/N98G | exhibits 0.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
H208A | exhibits about 1.4% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.1% compared to the wild-type value | Sphingobium herbicidovorans |
H272A | exhibits about 1.2% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
Q162F | exhibits about 4.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
R207A | exhibits about 17.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 2% compared to the wild-type value | Sphingobium herbicidovorans |
R207V | exhibits about 2.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate | Sphingobium herbicidovorans |
R274A | inactive mutant enzyme | Sphingobium herbicidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme E69A | Sphingobium herbicidovorans | |
0.161 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans | |
1.1 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme G97I/N98G | Sphingobium herbicidovorans | |
2.5 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R207A | Sphingobium herbicidovorans | |
2.9 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme H208A | Sphingobium herbicidovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium herbicidovorans | Q700X4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium herbicidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-dichlorprop. RdpA exclusively transforms the (S)-enantiomer of dichlorprop | Sphingobium herbicidovorans | 2,4-dichlorophenol + pyruvate + succinate + CO2 | - |
? | |
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 | i.e. (S)-mecoprop. RdpA exclusively transforms the (S)-enantiomer of mecoprop | Sphingobium herbicidovorans | 4-chloro-2-methylphenol + pyruvate + succinate + CO2 | - |
? | |
additional information | construction of homology models of SdpA and RdpA from Sphingomonas herbicidovorans MH and the use of docking to identify residues likely to be involved in herbicide binding | Sphingobium herbicidovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SdpA | - |
Sphingobium herbicidovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sphingobium herbicidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme H208A | Sphingobium herbicidovorans | |
0.7 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme G97I/N98G | Sphingobium herbicidovorans | |
3.97 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R207A | Sphingobium herbicidovorans | |
4 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme E69A | Sphingobium herbicidovorans | |
16.83 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Sphingobium herbicidovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme H208A | Sphingobium herbicidovorans | |
0.64 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme G97I/N98G | Sphingobium herbicidovorans | |
1.59 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme R207A | Sphingobium herbicidovorans | |
93.96 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, mutant enzyme E69A | Sphingobium herbicidovorans | |
104.5 | - |
(S)-2-(4-chloro-2-methylphenoxy)propanoate | pH 6.8, 30°C, wild-type enzyme | Sphingobium herbicidovorans |