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Literature summary for 1.14.11.41 extracted from

  • Helmetag, V.; Samel, S.A.; Thomas, M.G.; Marahiel, M.A.; Essen, L.O.
    Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis (2009), FEBS J., 276, 3669-3682.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.41

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Streptomyces vinaceus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor-diffusion method. The crystal structures of VioC are determined in complexes with the cofactor Fe(II), the substrate L-arginine, the product (2S,3S)-hydroxyarginine and the coproduct succinate at 1.1–1.3 A resolution Streptomyces vinaceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
3.4
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus
7.05
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Streptomyces vinaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41600
-
 x * 41600, SDS-PAGE Streptomyces vinaceus

Organism

Organism UniProt Comment Textmining
Streptomyces vinaceus Q6WZB0
-
-

Purification (Commentary)

Purification (Comment) Organism
expressed as a fusion protein with an N-terminal hexahistidine tag in Escherichia coli BL21(DE3) Streptomyces vinaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + 2-oxoglutarate + O2 L-arginine is the preferred substrate Streptomyces vinaceus (3S)-3-hydroxy-L-arginine + succinate + CO2
-
 ?
L-canavanine + 2-oxoglutarate + O2
-
 Streptomyces vinaceus ? + succinate + CO2
-
 ?
L-homoarginine + 2-oxoglutarate + O2
-
 Streptomyces vinaceus (3S)-N6-(diaminomethylidene)-3-hydroxy-L-lysine + succinate + CO2
-
 ?
additional information no activity with D-arginine, NG-methyl-L-arginine, NG-hydroxynor-L-arginine Streptomyces vinaceus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41600, SDS-PAGE Streptomyces vinaceus

Synonyms

Synonyms Comment Organism
VioC
-
 Streptomyces vinaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces vinaceus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.22
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
13.85
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus
43.5
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces vinaceus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the tuberactinomycin antibiotic viomycin Streptomyces vinaceus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.05
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
1.96
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus
12.8
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus