Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces hygroscopicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38600 | - |
x * 38600, protein with an N-terminal His6 tag, calculated from sequence | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-enduracididine + 2-oxoglutarate + O2 | Streptomyces hygroscopicus | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
L-enduracididine + 2-oxoglutarate + O2 | Streptomyces hygroscopicus NRRL 30439 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
- |
- |
Streptomyces hygroscopicus NRRL 30439 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-enduracididine + 2-oxoglutarate + O2 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
L-enduracididine + 2-oxoglutarate + O2 | MppO specifically hydroxylates L-enduracididine | Streptomyces hygroscopicus | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
L-enduracididine + 2-oxoglutarate + O2 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
L-enduracididine + 2-oxoglutarate + O2 | MppO specifically hydroxylates L-enduracididine | Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
additional information | no activity with L-arginine or dideoxy-mannopeptimycin-gamma | Streptomyces hygroscopicus | ? | - |
? | |
additional information | no activity with L-arginine or dideoxy-mannopeptimycin-gamma | Streptomyces hygroscopicus NRRL 30439 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38600, protein with an N-terminal His6 tag, calculated from sequence | Streptomyces hygroscopicus |
Synonyms | Comment | Organism |
---|---|---|
MppO | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces hygroscopicus |
General Information | Comment | Organism |
---|---|---|
malfunction | the role of MppO in beta-hydroxyenduracididine biosynthesis is confirmed through inactivation of mppO, which yields a strain that produces dideoxy-mannopeptimycins. MppO is responsible for introducing the beta-hydroxy group of both the D- and L-beta-hydroxyenduracididine residues | Streptomyces hygroscopicus |
physiological function | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus |