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Literature summary for 1.14.11.4 extracted from

  • Luther, K.B.; Huelsmeier, A.J.; Schegg, B.; Deuber, S.A.; Raoult, D.; Hennet, T.
    Mimivirus collagen is modified by bifunctional lysyl hydroxylase and glycosyltransferase enzyme (2011), J. Biol. Chem., 286, 43701-43709.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, and functional expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3) Acanthamoeba polyphaga Mimivirus

Protein Variants

Protein Variants Comment Organism
D250A site-directed mutagenesis, the mutant shows unaltered lysyl hydroxylase activity, although reduced collagen glucosyltransferase activity, compared to the wild-type enzyme Acanthamoeba polyphaga Mimivirus
D97A/D99A site-directed mutagenesis, the mutant cannot be expressed in Escherichia coli Acanthamoeba polyphaga Mimivirus
H80A site-directed mutagenes, the mutant cannot be expressed in Escherichia coli Acanthamoeba polyphaga Mimivirus
H825S/D827A site-directed mutagenesis, lysyl hydroxylase inactive mutant, that is still active as collagen glucosyltransferase Acanthamoeba polyphaga Mimivirus
L78K site-directed mutagenesis, the mutant cannot be expressed in Escherichia coli Acanthamoeba polyphaga Mimivirus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Acanthamoeba polyphaga Mimivirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine-[procollagen] + 2-oxoglutarate + O2 Acanthamoeba polyphaga Mimivirus
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 (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
 ?
L-lysine-[procollagen] + 2-oxoglutarate + O2 Acanthamoeba polyphaga Mimivirus L230
-
 (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
 ?
additional information Acanthamoeba polyphaga Mimivirus bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen ?
-
 ?
additional information Acanthamoeba polyphaga Mimivirus L230 bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acanthamoeba polyphaga Mimivirus
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-
-
Acanthamoeba polyphaga Mimivirus L230
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Acanthamoeba polyphaga Mimivirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GDK)4 + 2-oxoglutarate + O2 peptide acceptor substrate Acanthamoeba polyphaga Mimivirus ?
-
 ?
(GDK)4 + 2-oxoglutarate + O2 peptide acceptor substrate Acanthamoeba polyphaga Mimivirus L230 ?
-
 ?
L-lysine-[procollagen] + 2-oxoglutarate + O2
-
 Acanthamoeba polyphaga Mimivirus (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
 ?
L-lysine-[procollagen] + 2-oxoglutarate + O2
-
 Acanthamoeba polyphaga Mimivirus L230 (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
 ?
additional information bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen Acanthamoeba polyphaga Mimivirus ?
-
 ?
additional information the enzyme sequentially hydroxylates and glucosylates collagensubstrates in vitro Acanthamoeba polyphaga Mimivirus ?
-
 ?
additional information bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen Acanthamoeba polyphaga Mimivirus L230 ?
-
 ?
additional information the enzyme sequentially hydroxylates and glucosylates collagensubstrates in vitro Acanthamoeba polyphaga Mimivirus L230 ?
-
 ?

Synonyms

Synonyms Comment Organism
L230
-
 Acanthamoeba polyphaga Mimivirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Acanthamoeba polyphaga Mimivirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Acanthamoeba polyphaga Mimivirus

General Information

General Information Comment Organism
evolution whereas in animals from sponges to humans the transfer of galactose to hydroxylysine in collagen is conserved, the mimivirus L230 enzyme transfers glucose to hydroxylysine, thereby defining another type of collagen glycosylation in nature. Utilization of glucose instead of the galactose found throughout animals as well as a bifunctional enzyme rather than two separate enzymes may represent a parallel evolutionary track in collagen biology Acanthamoeba polyphaga Mimivirus