Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Homo sapiens | |
DTT | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged full-length JMJD6 and JMJD61-343 in Escherichia coli strain Rosetta | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged SeMet-derivatised and wild-type JMJD61-343, at both 20°C and 4°C by the sitting drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.75-2.0 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, binding site structure, overview | Homo sapiens | |
Ni2+ | can substitute for Fe2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant His-tagged catalytic domain of JMJD6, residues 1-343 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | JMJD6 catalyses the iron- and 2-oxoglutarate-dependent hydroxylation of lysyl residues in arginine-serine-rich domains of RNAsplicing-related proteins. It catalyses C5 hydroxylation rather than Nepsilon demethylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6NYC1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full-length JMJD6 and JMJD61-343 from Escherichia coli strain Rosetta by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luc7like2(267-278) + 2-oxoglutarate | substrate is a Luc7like2 protein fragment | Homo sapiens | ? + succinate + CO2 | - |
? | |
additional information | JMJD6 catalyses the iron- and 2-oxoglutarate-dependent hydroxylation of lysyl residues in arginine-serine-rich domains of RNAsplicing-related proteins. It catalyses C5 hydroxylation rather than Nepsilon demethylation | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant His-tagged catalytic domain of JMJD6, residues 1-343 | Homo sapiens |
More | structure of the catalytic domain of recombinant His-tagged JMJD6 and active site structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Jmjd6 | - |
Homo sapiens |
lysyl hydroxylase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |