Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star(DE3)pLysS cells | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GSTrap column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli W3110 / ATCC 27325 | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli W3110 / ATCC 27325 | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
methylated single-stranded poly(deoxyadenosine) + 2-oxoglutarate + O2 | - |
Escherichia coli | single-stranded poly(deoxyadenosine) + formaldehyde + succinate + CO2 | - |
? | |
methylated single-stranded poly(deoxyadenosine) + 2-oxoglutarate + O2 | - |
Escherichia coli W3110 / ATCC 27325 | single-stranded poly(deoxyadenosine) + formaldehyde + succinate + CO2 | - |
? | |
additional information | although the lesions targeted by AlkB are introduced primarily into single-stranded DNA, AlkB is also capable of removing lesions from double-stranded DNA | Escherichia coli | ? | - |
? | |
additional information | although the lesions targeted by AlkB are introduced primarily into single-stranded DNA, AlkB is also capable of removing lesions from double-stranded DNA | Escherichia coli W3110 / ATCC 27325 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | AlkB-mediated oxidative demethylation reverses DNA damage | Escherichia coli |