Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | purified AlkB repairs the cytotoxic lesion 1-methyladenine in single- and double-stranded DNA | Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | purified AlkB repairs the cytotoxic lesion 3-methylcytosine in single- and double-stranded DNA | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
methylated poly(deoxyadenine) + 2-oxoglutarate + O2 | 0.1 pmol AlkB protein removes 1.7 pmol 1-methyladenine from methylated poly(deoxyadenine) in 15 min | Escherichia coli | poly(deoxyadenine) + formaldehyde + succinate + CO2 | - |
? | |
methylated poly(deoxycytosine) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(deoxycytosine) + formaldehyde + succinate + CO2 | - |
? | |
additional information | AlkB has no detectable nuclease, DNA glycosylase or methyltransferase activity | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | Escherichia coli alkB mutants are defective in processing methylation damage generated in single-stranded DNA | Escherichia coli |
physiological function | oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage | Escherichia coli |