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Literature summary for 1.14.11.33 extracted from

  • Koivisto, P.; Duncan, T.; Lindahl, T.; Sedgwick, B.
    Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase (2003), J. Biol. Chem., 278, 44348-44354.
    View publication on PubMed
Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment) Organism
isoforms ABH2 and ABH3 are expressed in Sf9 insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 poly(dm1A) apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.002
-
 1-methyl-dAMP apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.0028
-
 d(Tpm1ApT) apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.0044
-
 d(Tpm1A) apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.0055
-
 1-methyl-dATP apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.01
-
 N1-methyl-ATP apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.012
-
 2'-deoxy-1-methyl-adenosine 3'-phosphate apparent value, at 37°C, pH not specified in the publication Escherichia coli
0.04 0.05 d(Tpm1A) apparent value, at 37°C, pH not specified in the publication Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Escherichia coli
Fe2+ required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-dAMP + 2-oxoglutarate + O2
-
 Escherichia coli dAMP + formaldehyde + succinate + CO2
-
 ?
1-methyl-dAMP + 2-oxoglutarate + O2 low activity Homo sapiens dAMP + formaldehyde + succinate + CO2
-
 ?
1-methyl-dATP + 2-oxoglutarate + O2
-
 Escherichia coli dATP + formaldehyde + succinate + CO2
-
 ?
1-methyl-dATP + 2-oxoglutarate + O2 low activity Homo sapiens dATP + formaldehyde + succinate + CO2
-
 ?
2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2
-
 Escherichia coli 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2
-
 ?
2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2 low activity Homo sapiens 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2
-
 ?
d(Tpm1A) + 2-oxoglutarate + O2
-
 Escherichia coli d(TpA) + formaldehyde + succinate + CO2
-
 ?
d(Tpm1A) + 2-oxoglutarate + O2 low activity Homo sapiens d(TpA) + formaldehyde + succinate + CO2
-
 ?
d(Tpm1ApT) + 2-oxoglutarate + O2
-
 Escherichia coli d(TpApT) + formaldehyde + succinate + CO2
-
 ?
d(Tpm1ApT) + 2-oxoglutarate + O2 low activity Homo sapiens d(TpApT) + formaldehyde + succinate + CO2
-
 ?
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
N1-methyl-ATP + 2-oxoglutarate + O2
-
 Escherichia coli ATP + formaldehyde + succinate + CO2
-
 ?
N1-methyl-ATP + 2-oxoglutarate + O2 low activity Homo sapiens ATP + formaldehyde + succinate + CO2
-
 ?
poly(dm1A) + 2-oxoglutarate + O2
-
 Escherichia coli poly(dA) + formaldehyde + succinate + CO2
-
 ?
poly(dm1A) + 2-oxoglutarate + O2 low activity Homo sapiens poly(dA) + formaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
1-methyladenine-DNA dioxygenase
-
 Escherichia coli
1-methyladenine-DNA dioxygenase
-
 Homo sapiens
ABH2 isoform Homo sapiens
ABH3 isoform Homo sapiens
AlkB
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 2'-deoxy-1-methyl-adenosine 3'-phosphate at 37°C, pH not specified in the publication Escherichia coli
0.02
-
 N1-methyl-ATP at 37°C, pH not specified in the publication Escherichia coli
0.033
-
 1-methyl-dATP at 37°C, pH not specified in the publication Escherichia coli
0.06
-
 d(Tpm1A) at 37°C, pH not specified in the publication Escherichia coli
0.07
-
 1-methyl-dAMP at 37°C, pH not specified in the publication Escherichia coli
0.12
-
 d(Tpm1ApT) at 37°C, pH not specified in the publication Escherichia coli
0.2
-
 poly(dm1A) at 37°C, pH not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Escherichia coli
2-oxoglutarate
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
 1-methyl-ATP at 37°C, pH not specified in the publication Escherichia coli
3.3
-
 2'-deoxy-1-methyl-adenosine 3'-phosphate at 37°C, pH not specified in the publication Escherichia coli
11.7
-
 1-methyl-dATP at 37°C, pH not specified in the publication Escherichia coli
35
-
 1-methyl-dAMP at 37°C, pH not specified in the publication Escherichia coli
43
-
 d(Tpm1ApT) at 37°C, pH not specified in the publication Escherichia coli
143
-
 poly(dm1A) at 37°C, pH not specified in the publication Escherichia coli