Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | AlkB uses a non-heme mononuclear iron(II) | Escherichia coli | |
Fe2+ | AlkB uses a non-heme mononuclear iron(II) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1,6-ethenoadenine + O2 | - |
Escherichia coli | DNA-adenine + glyoxal | - |
? | |
DNA-1,6-ethenoadenine + O2 | - |
Homo sapiens | DNA-adenine + glyoxal | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | best substrate for AlkB | Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | DNA-1-methyladenine is demethylated by isoforms ABH2 and ABH3 | Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methylguanine + 2-oxoglutarate + O2 | DNA-1-methylguanine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine | Escherichia coli | DNA-guanine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methylguanine + 2-oxoglutarate + O2 | DNA-1-methylguanine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine | Homo sapiens | DNA-guanine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | best substrate for AlkB | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | DNA-1-methylcytosine is demethylated by isoforms ABH2 and ABH3. Isoform ABH1 exclusively repairs DNA-3-methylcytosine in single-stranded DNA with low activity | Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | DNA-3-methylthymine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine | Escherichia coli | DNA-thymine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | DNA-3-methylthymine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine. Isoform FTO repairs DNA-3-methylthymine | Homo sapiens | DNA-thymine + formaldehyde + succinate + CO2 | - |
? | |
additional information | isoform ABH2 prefers double-stranded DNA while isoforms ABH3 and ABH1 work better on single-stranded substrates | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABH1 | isoform | Homo sapiens |
ABH2 | isoform | Homo sapiens |
ABH3 | isoform | Homo sapiens |
AlkB | - |
Escherichia coli |
FTO | isoform | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli | |
2-oxoglutarate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions | Escherichia coli |
physiological function | AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions | Homo sapiens |