Cloned (Comment) | Organism |
---|---|
phylogenetic tree, CODM real time quantitative PCR expression analysis, recombinant expression | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state enzyme kinetics of purified recombinant CODM using (S)-scoulerine as substrate | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + 2-oxoglutarate + O2 | Papaver somniferum | - |
morphine + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
cv. Bea's Choice | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-tetrahydropalmatine + 2-oxoglutarate + O2 | a protoberberine, O-demethylation, 16.6% of activity with protopine | Papaver somniferum | ? | - |
? | |
(S)-isocorydine + 2-oxoglutarate + O2 | a aporphine, O-demethylation, 6.1% of activity with protopine | Papaver somniferum | ? | - |
? | |
(S)-reticuline + 2-oxoglutarate + O2 | a 1-benzylisoquinoline, O-demethylation, 16.2% of activity with protopine | Papaver somniferum | ? | - |
? | |
(S)-scoulerine + 2-oxoglutarate + O2 | a 1-benzylisoquinoline, O-demethylation, 70.4% of activity with protopine | Papaver somniferum | ? | - |
? | |
allocryptopine + 2-oxoglutarate + O2 | a protopine, O-demethylation, 50.1% of activity with protopine | Papaver somniferum | ? | - |
? | |
codeine + 2-oxoglutarate + O2 | - |
Papaver somniferum | morphine + formaldehyde + succinate + CO2 | - |
? | |
codeine + 2-oxoglutarate + O2 | a morphinan, O-demethylation, 89.8% of activity with protopine | Papaver somniferum | morphine + formaldehyde + succinate + CO2 | - |
? | |
cryptopine + 2-oxoglutarate + O2 | a protopine, O-demethylation, 37.7% of activity with protopine | Papaver somniferum | ? | - |
? | |
O-demethylcryptopine + 2-oxoglutarate + O2 | a protopine, O-demethylation, 40.8% of activity with protopine | Papaver somniferum | ? | - |
? | |
protopine + 2-oxoglutarate + O2 | best substrate | Papaver somniferum | ? | - |
? | |
thebaine + 2-oxoglutarate + O2 | a morphinan, O-demethylation, 80.5% of activity with protopine | Papaver somniferum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CODM | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate/Fe(II)-dependent dioxygenase superfamily | Papaver somniferum |
metabolism | codeine O-demethylase catalyzes the last step of morphine biosynthesis in opium poppy | Papaver somniferum |
physiological function | codeine O-demethylase catalyzes the last step of morphine biosynthesis in opium poppy, but it also catalyze the O,O-demethylenation of methylenedioxy bridges on protopine alkaloids. No or poor activity with papaverine, (R,S)-tetrahydropapaverine, (R,S)-canadine, (R,S)-stylopine, berberine, and oripavine | Papaver somniferum |