Cloned (Comment) | Organism |
---|---|
His6-tagged protein is produced in Escherichia coli | Papaver somniferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
codeine | - |
Papaver somniferum | |
thebaine | - |
Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
2-oxoglutarate | pH 7.4, 30°C | Papaver somniferum | |
0.0205 | - |
codeine | pH 7.4, 30°C | Papaver somniferum | |
0.0419 | - |
thebaine | pH 7.4, 30°C | Papaver somniferum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Papaver somniferum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
high-morphine varieties (i.e. L, 11 and 40) and a mutant variety (i.e. T) that accumulates high levels of thebaine and oripavine, but lacks morphine and codeine | - |
Purification (Comment) | Organism |
---|---|
- |
Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + 2-oxoglutarate + O2 | alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde | Papaver somniferum | morphine + formaldehyde + succinate + CO2 | - |
? | |
additional information | no activity with (S)-reticuline, papaverine, (S)-corytuberine, pavine, salutaridine, oripavine, noscapine | Papaver somniferum | ? | - |
? | |
scoulerine + 2-oxoglutarate + O2 | 120% of the activity with codeine | Papaver somniferum | 3-O-demethylscoulerine + formaldehyde + succinate + CO2 | - |
? | |
thebaine + 2-oxoglutarate + O2 | 25% of the activity with codeine. Alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde | Papaver somniferum | oripavine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
codeine O-demethylase | - |
Papaver somniferum |
CODM | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0099 | - |
thebaine | pH 7.4, 30°C | Papaver somniferum | |
0.011 | - |
2-oxoglutarate | pH 7.4, 30°C | Papaver somniferum | |
0.0161 | - |
codeine | pH 7.4, 30°C | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Papaver somniferum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.642 | - |
codeine | pH 7.4, 30°C | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
malfunction | virus-induced gene silencing of CODM in opium poppy efficiently blocks metabolism at codeine | Papaver somniferum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
235.2 | - |
thebaine | pH 7.4, 30°C | Papaver somniferum | |
578.9 | - |
2-oxoglutarate | pH 7.4, 30°C | Papaver somniferum | |
785.4 | - |
codeine | pH 7.4, 30°C | Papaver somniferum |