Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES, 1.2 M (NH4)2SO4, and 3% PEG 400, pH 7.0 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D201A | inactive | Homo sapiens |
D201E | the mutant shows greatly impaired activity compared to the wild type enzyme | Homo sapiens |
D201G | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-oxoglutarate | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.023 | - |
2-oxoglutarate | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
0.026 | - |
2-oxoglutarate | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.07 | - |
O2 | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.07 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.071 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
0.078 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.115 | - |
O2 | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
0.2 | - |
O2 | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 | Homo sapiens | hydroxylation at Asn803 | hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NWT6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN + 2-oxoglutarate + O2 | - |
Homo sapiens | DESGLPQLTSYDAEV-(3S)-3-hydroxy-L-asparaginyl-APIQGSRNLLQGEELLRALDQVN + succinate + CO2 | - |
? | |
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 | hydroxylation at Asn803 | Homo sapiens | hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor inhibiting hypoxia inducible factor-1alpha | - |
Homo sapiens |
FIH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0135 | - |
O2 | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.027 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.33 | - |
O2 | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
0.383 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
0.55 | - |
O2 | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.55 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
O2 | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
0.37 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210E, at pH 7.0 and 37°C | Homo sapiens | |
2.83 | - |
O2 | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
3 | - |
O2 | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
5.7 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | mutant enzyme D210G, at pH 7.0 and 37°C | Homo sapiens | |
7 | - |
DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens |