Literature summary for 1.14.11.21 extracted from

Doan, L.X.; Hassan, A.; Lipscomb, S.J.; Dhanda, A.; Zhang, Z.; Schofield, C.J.
Mutagenesis studies on the iron binding ligands of clavaminic acid synthase (2000), Biochem. Biophys. Res. Commun., 279, 240-244.

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Protein Variants

Protein Variants	Comment	Organism
C162L	isozyme CAS2, site-directed mutagenesis, same activity in hydroxylation of N-alpha-acetyl-L-arginine as the wild-type enzyme	Streptomyces clavuligerus
C200L	isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
C201L	isozyme CAS2, site-directed mutagenesis, 40% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
C8L	isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H109L	isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H122L	isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H131L	isozyme CAS2, site-directed mutagenesis, 36% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H145E	isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H145L	isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H145Q	site-directed mutagenesis, no activity in hydroxylation of N-alpha-acetyl-L-arginine	Streptomyces clavuligerus
H151l	isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H167L	isozyme CAS2, site-directed mutagenesis, 19% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H280L	isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
H297L	isozyme CAS2, site-directed mutagenesis, 3% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus
Q154L	isozyme CAS2, site-directed mutagenesis, 23% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme	Streptomyces clavuligerus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required	Streptomyces clavuligerus
Fe2+	H145 forms part of the HXE iron-binding motif in isozyme CAS2	Streptomyces clavuligerus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	-	isozyme CAS2	-

Reaction

Reaction	Comment	Organism	Reaction ID
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O	reaction mechanism	Streptomyces clavuligerus	a
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O	reaction mechanism	Streptomyces clavuligerus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2	hydroxylation	Streptomyces clavuligerus	guanidinoproclavaminate + succinate + CO2 + H2O	-	?
dihydroclavaminate + 2-oxoglutarate + O2	cyclization	Streptomyces clavuligerus	clavaminate + succinate + CO2 + H2O	-	?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2	hydroxylation	Streptomyces clavuligerus	4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O	-	?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2	isozyme CAS2	Streptomyces clavuligerus	4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O	-	?
proclavaminate + 2-oxoglutarate + O2	saturation	Streptomyces clavuligerus	dihydroclavaminate + succinate + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CAS	-	Streptomyces clavuligerus

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Release 2023.1 (January 2023)