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Literature summary for 1.14.11.21 extracted from

  • Busby, R.W.; Townsend, C.A.
    A single monomeric iron center in clavaminate synthase catalyzes three nonsuccessive oxidative transformations (1996), Bioorg. Med. Chem., 4, 1059-1064.
    View publication on PubMed
Search for more literature for 1.14.11.21

Cloned(Commentary)

Cloned (Comment) Organism
isozymes CS1 and CS2, overexpression in Escherichia coli JM101 Streptomyces clavuligerus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ competitive Streptomyces clavuligerus
N-bromoacetyl-L-arginine isozyme CS2, inactivation, at 7.5 mM half-life of 11.2 min at 30°C, Co2+ competes, substrates protect Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Streptomyces clavuligerus
Fe2+ 1 ion per catalytically active enzyme molecule for the cyclization and the saturation reaction Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus this reaction is due to a proclavaminate amidinohydrolase, i.e. cla amidinoproclavaminate + succinate + CO2 + H2O
-
 ?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 clavaminate + succinate + CO2 + H2O
-
 ?
additional information Streptomyces clavuligerus biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor ?
-
 ?
proclavaminate + 2-oxoglutarate + O2 Streptomyces clavuligerus
-
 dihydroclavaminate + succinate + CO2 + H2O product is a transient intermediate in the overall reaction process ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
 isozyme CS2
-

Reaction

Reaction Comment Organism Reaction ID
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 this reaction is due to a proclavaminate amidinohydrolase PAH, gene cla Streptomyces clavuligerus
a
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 this reaction is due to a proclavaminate amidinohydrolase, i.e. cla Streptomyces clavuligerus amidinoproclavaminate + succinate + CO2 + H2O
-
 ?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
dihydroclavaminate + 2-oxoglutarate + O2 cyclization Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
additional information dependent on O2 and 2-oxoglutarate Streptomyces clavuligerus ?
-
 ?
additional information biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor Streptomyces clavuligerus ?
-
 ?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 isozyme CS2 Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
proclavaminate + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O product is a transient intermediate in the overall reaction process ?
proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O product is a transient intermediate in the overall reaction process ?

Synonyms

Synonyms Comment Organism
CS
-
 Streptomyces clavuligerus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
38.3
-
 N-bromoacetyl-L-arginine isozyme CS2, pH 7.0, 30°C Streptomyces clavuligerus