Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-ethynyl proclavaminate | noncompetitive | Streptomyces clavuligerus | |
4'-vinyl proclavaminate | noncompetitive | Streptomyces clavuligerus | |
4-cyclopropyl proclavaminate | noncompetitive | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
N-alpha-acetyl-L-arginine | pH 7.0, 22°C | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
strain ATCC 27064 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
65fold purified enzyme | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | amidinoproclavaminate + succinate + CO2 + H2O | 10% of the product is proclavaminate, 90% of the product is E-(2S)-5-amino-2(2'-oxoazetidin-1-yl)-pent-3,4-enoate | ? | |
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | isozyme CS2 | Streptomyces clavuligerus | E-(2S)-5-amino-2(2'-oxoazetidin-1-yl)-pent-3,4-enoate + succinate + CO2 + H2O + ? | 10% of the product is proclavaminate, 90% of the product is E-(2S)-5-amino-2(2'-oxoazetidin-1-yl)-pent-3,4-enoate | ? | |
dihydroclavaminate + 2-oxoglutarate + O2 | stereochemical course of oxygen insertion | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | specific for 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
additional information | 4'-vinyl proclavaminate, 4'-ethynyl proclavaminate, and 4-cyclopropyl proclavaminate are no substrates | Streptomyces clavuligerus | ? | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | isozyme CS2 | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | (4'RS)-rac-proclavaminate | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | stereochemical course of oxygen insertion | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, assay at | Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
N-alpha-acetyl-L-arginine | pH 7.0, 22°C | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces clavuligerus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki of 4'-vinyl proclavaminate, 4'-ethynyl proclavaminate, and 4-cyclopropyl proclavaminate is in the range of 2-10 mM | Streptomyces clavuligerus |