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Literature summary for 1.14.11.2 extracted from

  • Keskiaho, K.; Hieta, R.; Sormunen, R.; Myllyharju, J.
    Chlamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly (2007), Plant Cell, 19, 256-269.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cell Chlamydomonas reinhardtii

Protein Variants

Protein Variants Comment Organism
additional information suppression by RNAi leads to a defective cell wall consisting of a loose network of fibrils resembling the inner and outer W1 and W7-layers of the wild-type cell wall. The nine remaining P4H-like polypeptides of Chlamydomonas cannot fully compensate for the lack of isoform Cr-P4H-1 activity Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
Pyridine-2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
Pyridine-2,5-dicarboxylate
-
 Chlamydomonas reinhardtii
Zn2+
-
 Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 poly(L-Pro) Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2 Chlamydomonas reinhardtii
0.1
-
 (L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
0.1
-
 (L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
0.1
-
 (L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
0.12
-
 (L-Ser-L-Pro)19 L-proline
-
 Chlamydomonas reinhardtii
0.14
-
 poly(L-Pro) Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2 Chlamydomonas reinhardtii
0.25
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.5
-
 (Pro-Pro-Gly)10 L-proline
-
 Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Km value 0.03 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28315
-
 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii
29000
-
 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii
35000
-
 gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
 isoform Cr-P4H-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Ser-L-Pro)19 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro)19 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
(L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
(L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
(L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
(Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
ascorbate Km value 0.02 mM Chlamydomonas reinhardtii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 Zn2+
-
 Chlamydomonas reinhardtii
0.07
-
 Pyridine-2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
0.1
-
 Pyridine-2,5-dicarboxylate
-
 Chlamydomonas reinhardtii