Cloned (Comment) | Organism |
---|---|
co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2, in Sf9 cell | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | co-expression of PHY-4.1, product of alpha subunit-like gene Y43F8B.4, and beta-subunit protein-disulfide isomerase PDI2 results in active (PHY-4.1)2(PDI2)2 tetramers and in PHY-4.1/PDI2 dimers. Contrary to alpha subunits PHY-1, PHY-2, PHY-3, tetramers containing PHY-4.1 are able to hydroxylate poly(L-Pro) and other proline-rich peptides | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-oxoglutarate | complex of recombinant subunits PHY-4.1 and PDI2 | Caenorhabditis elegans | |
0.11 | - |
poly(L-Pro) | Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2 | Caenorhabditis elegans | |
0.22 | - |
poly(L-Pro) | Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2 | Caenorhabditis elegans | |
0.25 | - |
(L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | complex of recombinant subunits PHY-4.1 and PDI2 | Caenorhabditis elegans | |
2.5 | - |
(L-Pro-L-Pro-Gly)10 L-proline | complex of recombinant subunits PHY-4.1 and PDI2 | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.01 mM | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
PAGE | Caenorhabditis elegans |
180000 | - |
PAGE | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
PHY-4.1, product of alpha subunit-like gene Y43F8B.4 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant alpha subunit PHY4.1 is partly N-glycosylated | Caenorhabditis elegans |
proteolytic modification | sequence contains a GP67 signal peptide | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
(L-Pro-L-Pro-Gly)10 L-proline + 2-oxoglutarate + O2 | 44.8% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | Caenorhabditis elegans | (L-Pro-L-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | L-Ala-(L-Pro)4-Gly-L-Ile-L-Pro-Gly-L-Tyr-(L-Pro)2-L-Ala-(L-Pro)4-Gly-L-Val-Gly-(L-Pro)4-L-Gln-Gly trans-4-hydroxy-L-proline + succinate + CO2 | peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 380% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | ? | |
L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | L-Pro-L-Ile-(L-Pro)7-L-Leu-L-Pro-L-Gln-L-Asn-L-Leu-L-Ser-Gly-L-Ala-L-Pro trans-4-hydroxy-L-proline + succinate + CO2 | peptide substrate representing proline-rich proteins expressed in the pharyngeal gland cells. 610% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | ? | |
poly(L-Pro) + 2-oxoglutarate + O2 | Mr 30000-40000 Da, 15% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | Caenorhabditis elegans | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | Mr 5000-10000 Da, 5, 24% of the activity with (L-Pro-L-Glu-L-Pro-L-Pro-L-Ala)5 L-proline | Caenorhabditis elegans | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | and tetramer, 1 * alpha subunit PHY4.1 + 1 * beta subunit PDI2, SDS-PAGE of recombinant protein | Caenorhabditis elegans |
tetramer | and dimer, 2 * alpha subunit PHY4.1 + 2 * beta subunit PDI2, SDS-PAGE of recombinant protein | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | complex of recombinant subunits PHY-4.1 and PDI2, Km value 0.04 mM | Caenorhabditis elegans |