Crystallization (Comment) | Organism |
---|---|
structures of apoenzyme at 1.93 and 2.90 A resolution, complexed with the competitive inhibitor Zn2+, and with Zn2+ and pyridine 2,4-dicarboxylate at 1.85 A resolution. The structures reveal the double-stranded alpha-helix core fold typical for 2-oxoglutarate dioxygenases. The catalytic site is at the center of an extended shallow groove lined by two flexible loops | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
E127A | very low catalytic activity | Chlamydomonas reinhardtii |
H245A | no catalytic activity | Chlamydomonas reinhardtii |
Q130A | almost 2-fold increase in Km for poly(L-Pro) | Chlamydomonas reinhardtii |
R161A | no catalytic activity | Chlamydomonas reinhardtii |
R93A | no catalytic activity | Chlamydomonas reinhardtii |
S95A | no catalytic activity | Chlamydomonas reinhardtii |
W243A | Km values similar to wild-type, 10-fold decrease in kcat | Chlamydomonas reinhardtii |
W99A | 2.5-fold increase in Km for poly(L-Pro) | Chlamydomonas reinhardtii |
Y134F | 6-fold increase in Km for 2-oxoglutarate, decrease in kcat | Chlamydomonas reinhardtii |
Y140A | no catalytic activity | Chlamydomonas reinhardtii |
Y168F | 3-4-fold increase in Km for 2-oxoglutarate, decrease in kcat | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
2-oxoglutarate | mutant W99A | Chlamydomonas reinhardtii | |
0.14 | - |
2-oxoglutarate | mutant W243A | Chlamydomonas reinhardtii | |
0.15 | - |
2-oxoglutarate | mutant Q130A | Chlamydomonas reinhardtii | |
0.16 | - |
2-oxoglutarate | wild-type | Chlamydomonas reinhardtii | |
0.25 | - |
poly(L-Pro) | wild-type | Chlamydomonas reinhardtii | |
0.27 | - |
poly(L-Pro) | mutant Y168F | Chlamydomonas reinhardtii | |
0.28 | - |
poly(L-Pro) | mutant W243A | Chlamydomonas reinhardtii | |
0.28 | - |
poly(L-Pro) | mutant Y134F | Chlamydomonas reinhardtii | |
0.46 | - |
poly(L-Pro) | mutant Q130A | Chlamydomonas reinhardtii | |
0.57 | - |
2-oxoglutarate | mutant Y168F | Chlamydomonas reinhardtii | |
0.65 | - |
poly(L-Pro) | mutant W99A | Chlamydomonas reinhardtii | |
1 | - |
2-oxoglutarate | mutant Y134F | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(L-Pro) + 2-oxoglutarate + O2 | Mr of poly(L-Pro) is about 5000 | Chlamydomonas reinhardtii | poly(4-hydroxyproline) + succinate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
poly(L-Pro) | mutant Y134F | Chlamydomonas reinhardtii | |
6 | - |
poly(L-Pro) | mutant W243A | Chlamydomonas reinhardtii | |
20 | - |
poly(L-Pro) | mutant W99A | Chlamydomonas reinhardtii | |
30 | - |
poly(L-Pro) | mutant Y168F | Chlamydomonas reinhardtii | |
35 | - |
poly(L-Pro) | mutant Q130A | Chlamydomonas reinhardtii | |
70 | - |
poly(L-Pro) | wild-type | Chlamydomonas reinhardtii |