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Literature summary for 1.14.11.2 extracted from

  • Gorres, K.L.; Edupuganti, R.; Krow, G.R.; Raines, R.T.
    Conformational preferences of substrates for human prolyl 4-hydroxylase (2008), Biochemistry, 47, 9447-9455.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
poly(ethylene glycol)-Gly-L-Tyr-L-Pro-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens
1.6
-
poly(ethylene glycol)-Gly-L-Tyr-L-(2S,4S)-4-fluoroproline-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens
6.4
-
poly(ethylene glycol)-Gly-L-Tyr-(2S)-4-thiaproline-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron(IV)-oxo species in the active site Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoform P4H
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(ethylene glycol)-Gly-L-Tyr-(2S)-4-thiaproline-Gly-OCH2CH2OH + 2-oxoglutarate + O2 6% of the activity with L-prolyl peptide substrate Homo sapiens poly(ethylene glycol)-Gly-L-Tyr-L-(2S,4R)-thiaoxoproline-Gly-OCH2CH2OH + ? + CO2
-
?
poly(ethylene glycol)-Gly-L-Tyr-L-(2S,4S)-4-fluoroproline-Gly-OCH2CH2OH + 2-oxoglutarate + O2 26% of the activity with L-prolyl peptide substrate Homo sapiens poly(ethylene glycol)-Gly-L-Tyr-L-(2S)-4-oxoproline-Gly-OCH2CH2OH + ? + CO2
-
?
poly(ethylene glycol)-Gly-L-Tyr-L-Pro-Gly-OCH2CH2OH + 2-oxoglutarate + O2
-
Homo sapiens poly(ethylene glycol)-Gly-L-Tyr-L-(2S,4R)-4-hydroxy-Pro-Gly-OCH2CH2OH + succinate + CO2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
poly(ethylene glycol)-Gly-L-Tyr-L-(2S,4S)-4-fluoroproline-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens
6
-
poly(ethylene glycol)-Gly-L-Tyr-L-Pro-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens
7.8
-
poly(ethylene glycol)-Gly-L-Tyr-(2S)-4-thiaproline-Gly-OCH2CH2OH 30°C, pH 7.8 Homo sapiens