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Literature summary for 1.14.11.2 extracted from

  • Annunen, P.; Helaakoski, T.; Myllyharju, J.; Veijola, J.; Pihlajaniemi, T.; Kivirikko, K.I.
    Cloning of the human prolyl 4-hydroxylase a subunit isoform a(II) and characterization of the type II enzyme tetramer. The a(I) and a(II) subunits do not form a mixed a(I)a(II)b2 tetramer (1997), J. Biol. Chem., 272, 17342-17348.
    View publication on PubMed
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the alpha-II subunit, coexpression with the beta subunit in insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
poly(L-proline)
-
 Gallus gallus
poly(L-proline) type I und II enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 Protocollagen type I enzyme Homo sapiens
0.0011
-
 Protocollagen type II enzyme Homo sapiens
0.018
-
 (Pro-Pro-Gly)10 type I enzyme Homo sapiens
0.022
-
 2-oxoglutarate
-
 Gallus gallus
0.022
-
 2-oxoglutarate type I and type II enzyme Homo sapiens
0.095
-
 (Pro-Pro-Gly)10 type II enzyme Homo sapiens
7.8
-
 Gly-Val-Pro-Gly-Val type I enzyme Homo sapiens
22
-
 Gly-Val-Pro-Gly-Val type II enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Gallus gallus
Fe2+ pure type II enzyme: Km 0.006 mM, pure type I enzyme: Km 0.004 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens O15460
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant enzyme, using an affinity purification procedure based on the use of a histidine tag in the N terminus of the protein disulfide-isomerase/beta polypeptide Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain expression of enzyme alpha-I subunit mRNA Homo sapiens
-
calvarium embryo Gallus gallus
-
embryo
-
 Gallus gallus
-
fibroblast
-
 Homo sapiens
-
heart
-
 Gallus gallus
-
heart expression of enzyme alpha-I and alpha-II subunit mRNAs Homo sapiens
-
kidney
-
 Gallus gallus
-
kidney high expression of enzyme alpha-I subunit mRNA and low expression of enzyme alpha-II subunit mRNA Homo sapiens
-
liver
-
 Gallus gallus
-
liver high expression of enzyme alpha-I subunit mRNA, low expression of enzyme alpha-II subunit mRNA Homo sapiens
-
lung
-
 Gallus gallus
-
lung expression of enzyme alpha-I and alpha-II subunit mRNAs Homo sapiens
-
pancreas expression of enzyme alpha-I and alpha-II subunits mRNAs Homo sapiens
-
placenta expression of enzyme alpha-I and alpha-II subunits mRNAs Homo sapiens
-
skeletal muscle high expression of enzyme alpha-I subunit mRNA and low expression of enzyme alpha-II subunit mRNA Homo sapiens
-
sternum
-
 Gallus gallus
-
tendon
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus
additional information
-
 activities of recombinant type II and I enzymes in Triton X-100 extracts from insect cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Homo sapiens ? + succinate + CO2
-
 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
Gly-Val-Pro-Gly-Val + 2-oxoglutarate + O2
-
 Homo sapiens Gly-Val-4-hydroxyproline-Gly-Val + succinate + CO2
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 MW: 7000 and 44000 Homo sapiens poly(4-hydroxyproline) + succinate + CO2
-
 ?
protocollagen + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
protocollagen + 2-oxoglutarate + O2
-
 Homo sapiens 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer type II tetramer represents about 30% of the total enzyme Homo sapiens
tetramer type II tetramer represents about 5-15% in various tissues Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Gallus gallus
ascorbate pure type II enzyme: Km 0.34 mM, pure type I enzyme: Km 0.33 mM Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 poly(L-proline) MW: 44000, type I enzyme Homo sapiens
0.0005
-
 poly(L-proline) MW: 7000, type I enzyme Homo sapiens
0.02
-
 poly(L-proline) MW: 44000, type II enzyme Homo sapiens
0.095
-
 poly(L-proline) MW: 7000, type II enzyme Homo sapiens