Cloned (Comment) | Organism |
---|---|
cloning of the alpha-II subunit, coexpression with the beta subunit in insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
poly(L-proline) | - |
Gallus gallus | |
poly(L-proline) | type I und II enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
Protocollagen | type I enzyme | Homo sapiens | |
0.0011 | - |
Protocollagen | type II enzyme | Homo sapiens | |
0.018 | - |
(Pro-Pro-Gly)10 | type I enzyme | Homo sapiens | |
0.022 | - |
2-oxoglutarate | - |
Gallus gallus | |
0.022 | - |
2-oxoglutarate | type I and type II enzyme | Homo sapiens | |
0.095 | - |
(Pro-Pro-Gly)10 | type II enzyme | Homo sapiens | |
7.8 | - |
Gly-Val-Pro-Gly-Val | type I enzyme | Homo sapiens | |
22 | - |
Gly-Val-Pro-Gly-Val | type II enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus | |
Fe2+ | pure type II enzyme: Km 0.006 mM, pure type I enzyme: Km 0.004 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Homo sapiens | O15460 | - |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant enzyme, using an affinity purification procedure based on the use of a histidine tag in the N terminus of the protein disulfide-isomerase/beta polypeptide | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | expression of enzyme alpha-I subunit mRNA | Homo sapiens | - |
calvarium | embryo | Gallus gallus | - |
embryo | - |
Gallus gallus | - |
fibroblast | - |
Homo sapiens | - |
heart | - |
Gallus gallus | - |
heart | expression of enzyme alpha-I and alpha-II subunit mRNAs | Homo sapiens | - |
kidney | - |
Gallus gallus | - |
kidney | high expression of enzyme alpha-I subunit mRNA and low expression of enzyme alpha-II subunit mRNA | Homo sapiens | - |
liver | - |
Gallus gallus | - |
liver | high expression of enzyme alpha-I subunit mRNA, low expression of enzyme alpha-II subunit mRNA | Homo sapiens | - |
lung | - |
Gallus gallus | - |
lung | expression of enzyme alpha-I and alpha-II subunit mRNAs | Homo sapiens | - |
pancreas | expression of enzyme alpha-I and alpha-II subunits mRNAs | Homo sapiens | - |
placenta | expression of enzyme alpha-I and alpha-II subunits mRNAs | Homo sapiens | - |
skeletal muscle | high expression of enzyme alpha-I subunit mRNA and low expression of enzyme alpha-II subunit mRNA | Homo sapiens | - |
sternum | - |
Gallus gallus | - |
tendon | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
additional information | - |
activities of recombinant type II and I enzymes in Triton X-100 extracts from insect cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Homo sapiens | ? + succinate + CO2 | - |
? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Homo sapiens | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
Gly-Val-Pro-Gly-Val + 2-oxoglutarate + O2 | - |
Homo sapiens | Gly-Val-4-hydroxyproline-Gly-Val + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | MW: 7000 and 44000 | Homo sapiens | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Gallus gallus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Homo sapiens | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | type II tetramer represents about 30% of the total enzyme | Homo sapiens |
tetramer | type II tetramer represents about 5-15% in various tissues | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus | |
ascorbate | pure type II enzyme: Km 0.34 mM, pure type I enzyme: Km 0.33 mM | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
poly(L-proline) | MW: 44000, type I enzyme | Homo sapiens | |
0.0005 | - |
poly(L-proline) | MW: 7000, type I enzyme | Homo sapiens | |
0.02 | - |
poly(L-proline) | MW: 44000, type II enzyme | Homo sapiens | |
0.095 | - |
poly(L-proline) | MW: 7000, type II enzyme | Homo sapiens |