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Literature summary for 1.14.11.2 extracted from

  • Baader, E.; Tschank, G.; Baringhaus, K.H.; Burghard, H.; Guenzler, V.
    Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues (1994), Biochem. J., 300, 525-530.
No PubMed abstract available
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
dimethyloxalylalanine 50% inhibition in chicken embryo calvaria at 1 mM Gallus gallus
dimethyloxalylglycine 50% inhibition in chicken embryo calvaria at 0.002 mM, inhibitor of hydroxyproline synthesis in embryonic chicken lung Gallus gallus
oxalyl-beta-alanine competitive inhibition with respect to 2-oxoglutarate Gallus gallus
oxalylalanine inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.123 mM Gallus gallus
oxalylcystine competitive inhibition with respect to 2-oxoglutarate Gallus gallus
oxalylglycine inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.023 mM Gallus gallus
oxalylproline
-
 Gallus gallus
oxalylsarcosine noncompetitive inhibition with respect to 2-oxoglutarate Gallus gallus
oxalylvaline
-
 Gallus gallus
Pyridine 2,4-dicarboxylate
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome embryonic bone Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, affinity chromatography on poly-L-proline coupled to Sepharose 4B and DEAE-cellulose chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
bone embryo Gallus gallus
-
calvarium embryo Gallus gallus
-
embryo
-
 Gallus gallus
-
lung embryo Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0019 0.007 oxalylglycine
-
 Gallus gallus
0.04
-
 oxalylalanine
-
 Gallus gallus
1
-
 oxalylsarcosine
-
 Gallus gallus
1.25
-
 oxalylcystine
-
 Gallus gallus
1.4
-
 oxalyl-beta-alanine
-
 Gallus gallus
10
-
 oxalylproline value above Gallus gallus
10
-
 oxalylvaline value above Gallus gallus