Cloned (Comment) | Organism |
---|---|
cloning of the alpha subunit of the enzyme, expression of the alpha subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
poly(L-proline) | not inhibitory | Caenorhabditis elegans | |
Pyridine 2,4-dicarboxylate | recombinant enzyme, competitive inhibitor with respect to Fe2+ and 2-oxoglutarate | Caenorhabditis elegans | |
Zn2+ | recombinant enzyme, competitive inhibition with respect to Fe2+ and 2-oxoglutarate | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
(L-Pro-L-Pro-Gly)10 | recombinant enzyme | Caenorhabditis elegans | |
0.02 | - |
2-oxoglutarate | recombinant enzyme | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | recombinant enzyme: Km 0.005 mM | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
recombinant enzyme, gel filtration | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant enzyme, using anion exchange chromatography on a DEAE-cellulose column and two gel filtration steps | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Caenorhabditis elegans | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
poly(L-Pro) + 2-oxoglutarate + O2 | do not serve as substrate, assayed with recombinant enzyme | Caenorhabditis elegans | poly(4-hydroxyproline) + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | recombinant enzyme, alpha beta, ratio: 1 to 1 | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | recombinant enzyme, Km: 0.3 mM | Caenorhabditis elegans |